Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Doxycycline hydrochloride (Hyclate) 是一种四环素抗生素,是口服有效的广谱金属蛋白酶抑制剂,具有抗菌活性。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
2 mg | ¥ 125 | 5日内发货 | ||
10 mg | ¥ 258 | 5日内发货 | ||
25 mg | ¥ 413 | 5日内发货 | ||
50 mg | ¥ 663 | 5日内发货 |
Doxycycline hydrochloride 的其他形式现货产品:
产品描述 | Doxycycline hydrochloride (Hyclate) is a synthetic tetracycline derivative with similar antimicrobial activity. |
体外活性 | 在Wistar大鼠中,每天 Doxycycline(25 mg)能够阻断主动脉弹性蛋白的结构性恶化,降低动脉壁中基质金属蛋白酶MMP-2和MMP-9的活性,从而减少平滑肌细胞在内膜的复制. |
体内活性 | 在类风湿滑膜成纤维细胞和内皮细胞中,Doxycycline(50 μM)下调MMP-8的在mRNA和蛋白水平的表达,同时完全抑制II型胶原的降解。在角膜上皮细胞中,Doxycycline抑制脂多糖诱导的IL-1β的mRNA和蛋白的量的增加。 |
激酶实验 | Gelatin (0.1% (w/v) is added to standard LaemmLi acrylamide polymerization mixture. Tissue extract is mixed 1:2 with sample buffer [250 mM Tris-Cl pH 6.8, 10% (w/v) SDS, 20% (v/v) glycerol, 0.005% (w/v) bromphenol blue]. Serum is diluted 1:10 with electrophoresis buffer (2.5 mM Tris, 20 mM glycine, 0.005% SDS) and mixed 1:2 with sample buffer. Twenty μLs are loaded after 10-min incubation at room temperature without boiling. After electrophoresis at 90 V, the gels are soaked in 2.5% (w/v) Triton X-100, incubated 2 to 3 days at 37°C in gelatin digestion buffer [50 mM Tris-Cl, pH 8.0, 8 mM CaCl2, 10 mM ZnSO2, 0.02% (w/v) NaN3], stained in 0.05% Coomassie blue R-250 in acetic acid/methanol/water (1:4.5:4.5 by volume), destained in 10% acetic acid and 5% methanol, and scanned for lysis band intensity. The lysis band intensity is proportional to gelatinase activity and is quantified densitometrically by using One-Dimensional Scan software. The result, a number between 0.07 and 3.75, is normalized to the protein content by dividing the densitometry result with the relative optical density from the BCA protein assay kit result. The result is used for the analysis as the arbitrary unit. For the total MMP activity results of lysis bands of pro-MMP-9, active MMP-9, pro-MMP-2, and active MMP-2 are added. A protein size marker is used to determine the correct size. |
细胞实验 | Doxycycline is diluted in culture medium at a concentration of 10 μg/mL. All in vitro treatments are performed in SMC cultures at 90% confluence, when ECM synthesis in SMC starts to be evident. Doxycycline (20 nM) is diluted in culture medium at a concentration of 10 μg/mL (20 nM), at which no toxicity or variation in primary cultured SMC proliferation has been reported, as well as in other cell lines and the incubation time is 48 h. SMC-C and SMC-Ch are seeded at equal cell density in 6-well plates and, when confluence reaches 90%, 1 mL culture medium is added to each well containing 3.7×104?Bq L-[5-H3]-proline (9.62×1011 Bq/mmol). After 48?h incubation, cells are lysed with 0.5 mL 0.5 mol/L NaOH for 1 h. The resulting solution is neutralized with an equal amount of 0.5 mol/L HCl, and 50 μL are used to measure total proteins with the Bradford method. One volume of 10% TCA is added to the remaining 250 μL and centrifuged at 13,000 g for 15?min at 4°C. The resulting precipitate is dissolved in 100 μL 0.2 mol/L NaOH, and then neutralized with 1 mol/L HCl. The solution is incubated with collagenase buffer (Tris-HCl, pH 7.6 20 mM, and CaCl2 250 mM final concentration) and 10 units of collagenase at 37°C overnight. Then, 150 μL 10% TCA are added and centrifuged at 13000 g for 15?min at 4°C. The resulting supernatant is added to 4 mL of scintillation fluid and the radioactivity is measured in a liquid scintillation counter LS 600 TA. |
别名 | Doxylin, 盐酸多西环素, Vibramycin, Hyclate |
分子量 | 480.9 |
分子式 | C22H24N2O8·HCl |
CAS No. | 10592-13-9 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 10 mM
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.0794 mL | 10.3972 mL | 20.7943 mL | 51.9859 mL |
5 mM | 0.4159 mL | 2.0794 mL | 4.1589 mL | 10.3972 mL | |
10 mM | 0.2079 mL | 1.0397 mL | 2.0794 mL | 5.1986 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Doxycycline hydrochloride 10592-13-9 Microbiology/Virology Proteases/Proteasome MMP Antibacterial Antibiotic Inhibitor Matrix metalloproteinases Doxylin LNT-229 G55 inhibit 293T 盐酸多西环素 U343 Doxycycline anti-proliferative activity Doxycycline Hydrochloride MCF 12A Bacterial antibacterial activity Vibramycin Hyclate SVG inhibitor