目录号 | 产品详情 | 靶点 | |
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T73994 | |||
Nedosiran sodium 是一种靶向乳酸脱氢酶 (LDH)的 RNA 干扰剂 (RNAi)。Nedosiran sodium?可用于研究并发终末期肾病 (ESRD)的原发性高草酸尿症 (PH)。 | |||
T73500 | |||
AB-506 是一种具有口服活性的,靶向病毒核心蛋白 HBV 复制的抑制剂。AB-506 可以与 HBV 核心蛋白结合,加速衣壳组装并抑制 HBV pgRNA 包壳。AB-506 可用于慢性乙型肝炎 (CHB)研究。 | |||
T36881 | |||
NHC-triphosphate triammonium is an active phosphorylated intracellular metabolite of β-d-N4-Hydroxycytidine (NHC) as a triphosphate form[1]. NHC-triphosphate triammonium is a weak alternative substrate for the viral polymerase and can be incorporated into HCV replicon RNA[1][2]. In an intracellular metabolism assay, HCV replicon cells are treated with 10 μM 3H-labeled NHC, and intracellular nucleotide levels are determined after 1, 2 and 8 hours incubations. NHC is rapidly convered into the mono-, di-, and triphosphate forms, and NHC-TP reaches up to 71.12 pM after 8 hours[1].NHC-triphosphate triammonium (NHC-TP) (5-40 μM) absence leads to full-length polymerization products, it can be a weak alternative substrate. In addition, incorporation of NHC-TP instead of CTP increases the molecular weight of the polymerization product by 16 (one extra oxygen) for each event and an obvious electrophoretic shift is observed in cell-free HCV NS5B polymerization reactions[1].Huh-7 cells are incubated with (10-50 μM; 4 h) NHC or a McGuigan phosphoramidate prodrug of NHC. Intracellular levels of the parental compounds and phosphorylated metabolites are measured using LC-MS/MS. Small amounts of NHC-monophosphate (MP) and NHC-diphosphate (DP) can be observed, while NHC-triphosphate triammonium remains the most abundant metabolite[2].NHC-triphosphate triammonium (NHC-TP) metabolite may directly target the viral polymerase and behave as a nonobligate chain terminator. It plays a prominent role in inhibiting early negative-strand RNA synthesis, either through chain termination or mutagenesis, which may in turn interfere with correct replicase complex formation. [1]. Stuyver LJ,et al. Ribonucleoside analogue that blocks replication of bovine viral diarrhea and hepatitis C viruses in culture.Antimicrob Agents Chemother. 2003 Jan;47(1):244-54. [2]. Maryam Ehteshami, et al. Characterization of β-d- N4-Hydroxycytidine as a Novel Inhibitor of Chikungunya Virus. | |||
T76193 | |||
PINT-87aa是一种由LINC-PINT编码的环状RNAp53诱导的转录本所编码的87氨基酸肽。该肽能直接与PAF1c互作,抑制多种癌基因的转录延伸,并在体外及体内抑制胶质母细胞瘤细胞增殖。 | |||
T75114 | |||
CD19car mRNA-LNP是一种脂质纳米粒(LNP),内含CD19car mRNA,用于RNA传递、翻译效率和细胞活力检测。该产品应用于嵌合抗原受体T细胞免疫疗法(CAR-CD19),CD19car作为嵌合抗原受体,针对B细胞表面的CD分子(即白细胞分化抗原)CD19,该分子在B细胞增殖、分化、活化及抗体产生中发挥关键作用,并促进BCR信号转导。 | |||
T38328 | |||
CU-32 is an inhibitor of cyclic GMP-AMP (cGAMP) synthase (cGAS; IC50= 0.45 μM).1It reduces DNA-, but not Sendai virus-, induced dimerization of IFN regulatory factor 3 in THP-1 cells, indicating selectivity for the cGAS DNA sensing pathway over the RIG-I-MAVS RNA sensing pathway. It is also selective for cGAS over toll-like receptors (TLRs) at 50 μM. CU-32 decreases IFN-stimulatory DNA-induced production of IFN-β in THP-1 cells when used at concentrations of 10, 30, and 100 μM. 1.Padilla-Salinas, R., Sun, L., Anderson, R., et al.Discovery of small-molecule cyclic GMP-AMP synthase inhibitorsJ. Org. Chem.85(3)1579-1600(2020) | |||
T35594 | |||
The Survival of Motor Neurons (SMN) protein participates in RNA splicing. Decreases in SMN, typically a consequence of defects in the smn1 gene, result in the death of motor neurons and lead to the neurodegenerative disease, spinal muscular atrophy (SMA). Cuspin-1 is a small molecule upregulator of SMN that has been shown in vitro to increase levels of SMN in SMA patient fibroblasts by 50% at 18 μM. Its mechanism of action is thought to involve increased phosphorylation of ERK to initiate Ras-Raf-MEK signaling, which results in an increased rate of SMN translation. | |||
T78526 | Others | ||
4A3-SC8为一种模块化降解型树突状分子,其功能是在侵袭性肝癌模型中使小RNA延长生存期。 | |||
T32091 | |||
Hoffman's violet is specific stains for animal chromosomes. Iodinated alcohol after staining can be substituted with various acids, both organic as well as inorganic, all of which act as trapping agent preventing leaching of the dye that binds with the ch | |||
T83964 | |||
tri-GalNAc-PEG3-Azide是一种用于溶酶体靶向嵌合体(LYTAC)研究与开发的功能化无乙酰神经氨酸糖蛋白受体(ASGPR)配体;每个分子包括三个ASGPR配体、一个PEG3连接器和一个准备用于接合的活性叠氮基团。绑定到ASGPR后,tri-GalNAc结合物通过ASGPR介导的内吞作用高效地被内化。tri-GalNAc接合可作为一种策略,以细胞特异性方式有效地将如RNA或Cas9复合体等货物递送至肝细胞。可用于生成LYTACs,或用染料标记以进行组织成像。 |
目录号 | 产品名/同用名 | 种属 | 表达系统 | ||
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TMPY-06071 | SARS-CoV-2 RNA-dependent RNA polymerase/RDRP Protein (His) | SARS-CoV-2 | Baculovirus Insect Cells | ||
SARS-CoV-2 RNA-dependent RNA polymerase/RDRP Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 108.3 kDa and the accession number is YP_009725307.1.
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TMPY-03983 | CIRBP Protein, Human, Recombinant (His) | Human | E. coli | ||
CIRBP, also known as cold-inducible RNA-binding protein, plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. CIRBP responds to a wide array of cellular stresses, including short wavelength ultraviolet light (UVC), at the transcriptional and post-translational level. It acts as a translational activator.CIRBP can bind the 3 translated region of specific transcripts to stabilize them and facilitate their transport to ribosomes for translation. CIRBP affects NF-κB signaling as opposed to IL1B mRNA stability directly.
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TMPH-01110 | CIRBP Protein, Human, Recombinant (GST) | Human | E. coli | ||
CIRBP Protein, Human, Recombinant (GST) is expressed in E. coli.
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TMPH-02587 | CIRBP Protein, Mouse, Recombinant (His) | Mouse | E. coli | ||
Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Promotes assembly of stress granules (SGs), when overexpressed. Seems to play an essential role in cold-induced suppression of cell proliferation. Acts as a translational repressor. Acts as a translational activator. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN.
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TMPH-03193 | Alba Protein, Pyrococcus furiosus, Recombinant (His & SUMO) | Pyrococcus furiosus | E. coli | ||
Binds double-stranded DNA tightly but without sequence specificity. It is distributed uniformly and abundantly on the chromosome, suggesting a role in chromatin architecture. However, it does not significantly compact DNA. Binds rRNA and mRNA in vivo. May play a role in maintaining the structural and functional stability of RNA, and, perhaps, ribosomes. Alba Protein, Pyrococcus furiosus, Recombinant (His & SUMO) is expressed in E. coli expression system with N-6xHis-SUMO tag. The predicted molecular weight is 26.4 kDa and the accession number is Q8TZV1.
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TMPH-01900 | DDX5 Protein, Human, Recombinant (His & Myc) | Human | E. coli | ||
DDX5 Protein, Human, Recombinant (His & Myc) is expressed in E. coli expression system with N-10xHis and C-Myc tag. The predicted molecular weight is 74.1 kDa and the accession number is P17844.
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TMPH-01248 | ADAR Protein, Human, Recombinant (His & SUMO) | Human | E. coli | ||
Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins; pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication; and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2) and serotonin (HTR2C) and GABA receptor (GABRA3). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alters their functional activities. Exhibits low-level editing at the GRIA2 Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Its viral RNA substrates include: hepatitis C virus (HCV), vesicular stomatitis virus (VSV), measles virus (MV), hepatitis delta virus (HDV), and human immunodeficiency virus type 1 (HIV-1). Exhibits either a proviral (HDV, MV, VSV and HIV-1) or an antiviral effect (HCV) and this can be editing-dependent (HDV and HCV), editing-independent (VSV and MV) or both (HIV-1). Impairs HCV replication via RNA editing at multiple sites. Enhances the replication of MV, VSV and HIV-1 through an editing-independent mechanism via suppression of EIF2AK2/PKR activation and function. Stimulates both the release and infectivity of HIV-1 viral particles by an editing-dependent mechanism where it associates with viral RNAs and edits adenosines in the 5'UTR and the Rev and Tat coding sequence. Can enhance viral replication of HDV via A-to-I editing at a site designated as amber/W, thereby changing an UAG amber stop codon to an UIG tryptophan (W) codon that permits synthesis of the large delta antigen (L-HDAg) which has a key role in the assembly of viral particles. However, high levels of ADAR1 inhibit HDV replication.
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TMPH-01249 | ADAR Protein, Human, Recombinant (GST) | Human | E. coli | ||
ADAR Protein, Human, Recombinant (GST) is expressed in E. coli expression system with N-GST tag. The predicted molecular weight is 47.2 kDa and the accession number is P55265.
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TMPH-02133 | DDX39B Protein, Human, Recombinant (GST) | Human | E. coli | ||
Involved in nuclear export of spliced and unspliced mRNA. Assembling component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. May undergo several rounds of ATP hydrolysis during assembly of TREX to drive subsequent loading of components such as ALYREF/THOC and CHTOP onto mRNA. Also associates with pre-mRNA independent of ALYREF/THOC4 and the THO complex. Involved in the nuclear export of intronless mRNA; the ATP-bound form is proposed to recruit export adapter ALYREF/THOC4 to intronless mRNA; its ATPase activity is cooperatively stimulated by RNA and ALYREF/THOC4 and ATP hydrolysis is thought to trigger the dissociation from RNA to allow the association of ALYREF/THOC4 and the NXF1-NXT1 heterodimer. Involved in transcription elongation and genome stability.; Splice factor that is required for the first ATP-dependent step in spliceosome assembly and for the interaction of U2 snRNP with the branchpoint. Has both RNA-stimulated ATP binding/hydrolysis activity and ATP-dependent RNA unwinding activity. Even with the stimulation of RNA, the ATPase activity is weak. Can only hydrolyze ATP but not other NTPs. The RNA stimulation of ATPase activity does not have a strong preference for the sequence and length of the RNA. However, ssRNA stimulates the ATPase activity much more strongly than dsRNA. Can unwind 5' or 3' overhangs or blunt end RNA duplexes in vitro. The ATPase and helicase activities are not influenced by U2AF2; the effect of ALYREF/THOC4 is reported conflictingly with [PubMed:23299939] reporting a stimulatory effect.; (Microbial infection) The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production.
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TMPY-03344 | LSM3 Protein, Human, Recombinant (His) | Human | E. coli | ||
LSM3 (LSM3 Homolog, U6 Small Nuclear RNA And MRNA Degradation Associated) is a Protein Coding gene. LSM3 is a member of the snRNP Sm proteins family. It plays role in pre-mRNA splicing as a component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as a component of the pre-catalytic spliceosome (spliceosome B complex). Sm-like proteins can be detected in a variety of organisms. They all have the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing. Diseases associated with LSM3 include Spondylolysis.
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TMPH-00974 | DHX9 Protein, Human, Recombinant (His & SUMO) | Human | E. coli | ||
DHX9 Protein, Human, Recombinant (His & SUMO) is expressed in E. coli.
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TMPH-00533 | T7 RNA polymerase Protein, Enterobacteria phage T7, Recombinant (His & Myc) | Escherichia phage T7 | E. coli | ||
Highly processive DNA-dependent RNA polymerase that catalyzes the transcription of class II and class III viral genes. Recognizes a specific promoter sequence and enters first into an 'abortive phase' where very short transcripts are synthesized and released before proceeding to the processive transcription of long RNA chains. Unwinds the double-stranded DNA to expose the coding strand for templating. Participates in the initiation of viral DNA replication presumably by making primers accessible to the DNA polymerase, thus facilitating the DNA opening. Plays also a role in viral DNA packaging, probably by pausing the transcription at the right end of concatemer junction to allow packaging complex recruitment and beginning of the packaging process.
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TMPH-01902 | DDX60 Protein, Human, Recombinant (His) | Human | E. coli | ||
Positively regulates DDX58/RIG-I- and IFIH1/MDA5-dependent type I interferon and interferon inducible gene expression in response to viral infection. Binds ssRNA, dsRNA and dsDNA and can promote the binding of DDX58/RIG-I to dsRNA. Exhibits antiviral activity against hepatitis C virus and vesicular stomatitis virus (VSV). DDX60 Protein, Human, Recombinant (His) is expressed in E. coli expression system with N-6xHis tag. The predicted molecular weight is 23.1 kDa and the accession number is Q8IY21.
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TMPH-00980 | DHX33 Protein, Human, Recombinant (His) | Human | E. coli | ||
DHX33 Protein, Human, Recombinant (His) is expressed in E. coli.
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TMPH-00979 | DDX3Y Protein, Human, Recombinant (His & Myc) | Human | Baculovirus Insect Cells | ||
DDX3Y Protein, Human, Recombinant (His & Myc) is expressed in Baculovirus.
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TMPH-00976 | DDX19A Protein, Human, Recombinant (His & SUMO) | Human | E. coli | ||
DDX19A Protein, Human, Recombinant (His & SUMO) is expressed in E. coli.
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TMPH-02884 | CTDP1 Protein, Mouse, Recombinant (His & Myc) | Mouse | E. coli | ||
Processively dephosphorylates 'Ser-2' and 'Ser-5' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit. This promotes the activity of RNA polymerase II. Plays a role in the exit from mitosis by dephosphorylating crucial mitotic substrates (USP44, CDC20 and WEE1) that are required for M-phase-promoting factor (MPF)/CDK1 inactivation. CTDP1 Protein, Mouse, Recombinant (His & Myc) is expressed in E. coli expression system with N-10xHis and C-Myc tag. The predicted molecular weight is 24.0 kDa and the accession number is Q7TSG2.
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TMPH-01556 | EIF2AK2 Protein, Human, Recombinant (His) | Human | P. pastoris (Yeast) | ||
EIF2AK2 Protein, Human, Recombinant (His) is expressed in yeast with N-6xHis tag. The predicted molecular weight is 64.0 kDa and the accession number is P19525.
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TMPH-00973 | DHX36 Protein, Human, Recombinant (His & Myc) | Human | E. coli | ||
DHX36 Protein, Human, Recombinant (His & Myc) is expressed in E. coli expression system with N-10xHis and C-Myc tag. The predicted molecular weight is 18.2 kDa and the accession number is Q9H2U1.
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TMPH-00975 | DDX1 Protein, Human, Recombinant (His & Myc) | Human | E. coli | ||
DDX1 Protein, Human, Recombinant (His & Myc) is expressed in E. coli.
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TMPH-01899 | ALKBH5 Protein, Human, Recombinant (His) | Human | E. coli | ||
ALKBH5 Protein, Human, Recombinant (His) is expressed in E. coli expression system with N-6xHis tag. The predicted molecular weight is 48.2 kDa and the accession number is Q6P6C2.
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TMPH-01310 | EIF3G Protein, Human, Recombinant (His & SUMO) | Human | E. coli | ||
EIF3G Protein, Human, Recombinant (His & SUMO) is expressed in E. coli expression system with N-6xHis-SUMO tag. The predicted molecular weight is 51.5 kDa and the accession number is O75821.
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TMPH-00977 | DDX3X Protein, Human, Recombinant (B2M & His) | Human | E. coli | ||
DDX3X Protein, Human, Recombinant (B2M & His) is expressed in E. coli.
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TMPH-01901 | DDX53 Protein, Human, Recombinant (His & Myc) | Human | E. coli | ||
N/A. DDX53 Protein, Human, Recombinant (His & Myc) is expressed in E. coli expression system with N-10xHis and C-Myc tag. The predicted molecular weight is 76.2 kDa and the accession number is Q86TM3.
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TMPH-00978 | DDX3X Protein, Human, Recombinant (His & Myc) | Human | Baculovirus Insect Cells | ||
DDX3X Protein, Human, Recombinant (His & Myc) is expressed in Baculovirus insect cells with N-10xHis and C-Myc tag. The predicted molecular weight is 77 kDa and the accession number is O00571.
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TMPH-01036 | Caprin-1/CAPRIN1 Protein, Human, Recombinant (His) | Human | Baculovirus Insect Cells | ||
May regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Binds directly and selectively to MYC and CCND2 RNAs. In neuronal cells, directly binds to several mRNAs associated with RNA granules, including BDNF, CAMK2A, CREB1, MAP2, NTRK2 mRNAs, as well as to GRIN1 and KPNB1 mRNAs, but not to rRNAs. Caprin-1/CAPRIN1 Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with N-10xHis tag. The predicted molecular weight is 80.7 kDa and the accession number is Q14444.
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TMPY-04870 | Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 protein (His) | ZIKV | HEK293 Cells | ||
Zika virus NS1 antigen is one of seven non-structural proteins. NS1 is involved in RNA replication. The possible effects of NS1 on hosts include: localizes to host cell surface and secreted extracellularly, modulates signalling of the innate immune system, has possible damages to platelets and endothelial cells through anti-NS1 antibodies.
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TMPK-00265 | SARS-COV-2 Nucleocapsid Protein (His & Avi), Biotinylated | SARS-CoV-2 | E. coli | ||
Nucleocapsid protein (N) is the major viral structural component; its main function is to protect and encapsidate the viral RNA forming viral RNP complex. It is encoded by the S segment vRNA and is abundantly expressed in the cytoplasm of infected cells. SARS-COV-2 Nucleocapsid Protein (His & Avi), Biotinylated is expressed in E. coli expression system with N-His-Avi tag. The predicted molecular weight is 48.9 kDa and the accession number is P0DTC9.
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TMPY-05822 | Hepatitis B Virus (HBV)(ayw/France/Tiollais/1979) Capsid protein (His) | HBV-D | E. coli | ||
Hepatitis B virus (HBV) capsid assembly is a critical step in the propagation of the virus and is mediated by the core protein. The first cytoplasmic step in the formation of an infectious HBV virion is the formation of a capsid containing pregenomic RNA (pgRNA) and the viral polymerase (Pol). HBV capsid assembly is an attractive target for new antiviral therapies.
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TMPY-02219 | Influenza A H1N1 (A/Puerto Rico/8/34/Mount Sinai) Non-structural/NS1 Protein (His) | H1N1 | E. coli | ||
The NS1 Influenza protein is created by the internal protein-encoding, linear negative-sense, single-stranded RNA, NS gene segment and which also codes for the nuclear export protein or NEP, formerly referred to as the NS2 protein, which mediates the export of vRNPs. The non-structural (NS1) protein is found in Influenzavirus A, Influenzavirus B, and Influenzavirus C. The non-structural (NS1) protein of the highly pathogenic avian H5N1 viruses circulating in poultry and waterfowl in Southeast Asia is currently believed to be responsible for the enhanced virulence of the strain. The Non-structural (NS1) protein of influenza A virus is a non-essential virulence factor that has multiple accessory functions during viral infection. The major role ascribed to NS1 has been its inhibition of host immune responses, especially the limitation of both interferon (IFN) production and the antiviral effects of IFN-induced proteins, such as dsRNA-dependent protein kinase R (PKR) and 2'5'-oligoadenylate synthetase (OAS)/RNase L. Non-structural (NS1) protein is a non-structural protein of the influenza A virus, which could only be expressed when cells are infected. The effect of NS1 protein on the host cell is still not clear. Not only could NS1 remarkably affect metabolism, but it could also slow down cell proliferation by blocking the cell cycle. Non-structural (NS1) protein may lead to the development of novel antiviral drugs, and the use of oncolytic influenza A viruses as potential anti-cancer agents.
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TMPY-05024 | Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS5 protein (His) | ZIKV | Baculovirus Insect Cells | ||
Zika virus NS5 is involved in methytransferase and RNA guanylytransferase activities and capping and synthesis of RNA. And, NS5 is also an RNA-dependent RNA polymerase.
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TMPY-05749 | SARS-CoV-2 NSP8 Protein (Avi) | SARS-CoV-2 | E. coli | ||
NSP8 is a nonstructural protein of coronavirus. NSP8 acts as a primase in RNA synthesis. NSP8 and NSP7 are essential co-factors of NSP12 (the catalytic subunit with RNA-dependent RNA polymerase activity) that can remarkably stimulate RdRp activity. The nsp12-nsp7-nsp8 subcomplex is defined as the minimal core component for mediating coronavirus RNA synthesis. SARS-CoV-2 NSP8 Protein (Avi) is expressed in E. coli expression system. The predicted molecular weight is 24.17 kDa and the accession number is YP_009725304.1.
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TMPH-01674 | Human metapneumovirus (strain CAN97-83) Nucleoprotein/NP Protein (His & Myc) | HMPV | E. coli | ||
Encapsidates the viral RNA genome by forming a left-handed helical nucleocapsid that protects the RNA from nucleases. RNA replication depends on the availability of soluble nucleoprotein. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication.
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TMPH-00811 | Hepatitis delta virus genotype I (HDV) Small delta antigen Protein (His & Myc) | HDV | P. pastoris (Yeast) | ||
Promotes both transcription and replication of genomic RNA. Following virus entry into host cell, provides nuclear import of HDV RNPs thanks to its nuclear localization signal. May interact with host RNA polymerase II thereby changing its template requirement from DNA to RNA. RNA pol II complex would then acts as an RNA-directed RNA polymerase, and transcribe and replicate HDV genome. Hepatitis delta virus genotype I (HDV) Small delta antigen Protein (His & Myc) is expressed in yeast with N-6xHis and C-Myc tag. The predicted molecular weight is 25.4 kDa and the accession number is P06934.
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TMPH-02380 | RPB5 Protein, Kluyveromyces marxianus, Recombinant (His) | Kluyveromyces marxianus | E. coli | ||
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process.
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TMPH-00732 | RtcB Protein, E. coli, Recombinant (His) | E. coli | P. pastoris (Yeast) | ||
GTP-dependent RNA ligase that is involved in tRNA splicing and RNA repair. Joins RNA with 2',3'-cyclic-phosphate or 3'-phosphate ends to RNA with 5'-hydroxy ends. Also acts as a DNA ligase in case of DNA damage by splicing 'dirty' DNA breaks, characterized by 3'-phosphate (or cyclic-phosphate) and 5'-hydroxy ends that cannot be sealed by classical DNA ligases.
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TMPH-03729 | Zaire ebolavirus (strain Kikwit-95) VP30 Protein (His) | ZEBOV | E. coli | ||
Multifunctional protein that acts as a viral transcriptional activator. Promotes read-through of an RNA hairpin in the NP open reading frame to enhance viral transcription. Mechanistically, nonphosphorylated VP30 hexamers form a ternary complex with the viral leader RNA. Clamps the RNA template and the complex VP35-polymerase L together, thereby increasing the polymerase affinity for the RNA template to increase transcription initiation despite the presence of RNA secondary structures. Assists also stop-start transcription at gene junctions to promote transcription of downstream genes. Interaction with NP plays a critical role in transcription initiation by recognizing the RNA stem loop. Interaction with host RBBP6 interferes with NP-VP30 interaction and inhibits viral RNA synthesis. Also acts as a suppressor of RNA silencing by interacting with host DICER1 and TARBP2/TRBP.
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TMPH-03184 | P2 Protein, Pseudomonas phage phi6, Recombinant (His & Myc) | Pseudomonas phage phi6 | E. coli | ||
Rna-dependent RNA polymerase part of the packaging complex that packages the viral RNA segments, replicate them into a double-stranded form and transcribe them. P2 Protein, Pseudomonas phage phi6, Recombinant (His & Myc) is expressed in E. coli expression system with N-10xHis and C-Myc tag. The predicted molecular weight is 27.2 kDa and the accession number is P11124.
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TMPH-03732 | Zaire ebolavirus (strain Kikwit-95) VP35 Protein (E. col, His & Myc) | ZEBOV | E. coli | ||
Plays an essential role in viral RNA synthesis and also a role in suppressing innate immune signaling. Acts as a polymerase cofactor in the RNA polymerase transcription and replication complexes. Serves as nucleoprotein/NP monomer chaperone prior to the formation of the large oligomeric RNA-bound complexes. Regulates RNA synthesis by modulating NP-RNA interactions and interacting with DYNLL1. VP35-NP interaction controls the switch between RNA-bound NP and free NP and thus the switch between genome replication and genome packaging into the nucleocapsid. Prevents establishment of cellular antiviral state, thereby suppressing host DC maturation. Acts by inhibiting host DDX58/RIG-I activation both by shielding dsRNA from detection and by preventing PRKRA binding to DDX58. Blocks virus-induced phosphorylation and activation of interferon regulatory factor 3/IRF3, a transcription factor critical for the induction of interferons alpha and beta. This blockage is produced through the interaction with and inhibition of host IKBKE and TBK1, producing a strong inhibition of the phosphorylation and activation of IRF3. Also inhibits the antiviral effect mediated by the host interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR. Increases PIAS1-mediated SUMOylation of IRF7, thereby repressing interferon transcription. Also acts as a suppressor of RNA silencing by interacting with host DICER1, TARBP2/TRBP and PRKRA/PACT. As a dimer, binds and sequesters dsRNA contributing to the inhibition of interferon production.
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TMPY-06416 | SARS-CoV-2 NSP8 Protein | SARS-CoV-2 | E. coli | ||
NSP8 is a nonstructural protein of coronavirus. NSP8 acts as a primase in RNA synthesis. NSP8 and NSP7 are essential co-factors of NSP12 (the catalytic subunit with RNA-dependent RNA polymerase activity) that can remarkably stimulate RdRp activity. The nsp12-nsp7-nsp8 subcomplex is defined as the minimal core component for mediating coronavirus RNA synthesis. SARS-CoV-2 NSP8 Protein is expressed in E. coli expression system. The predicted molecular weight is 22.04 kDa and the accession number is YP_009725304.1.
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TMPH-03713 | ELAVL4 Protein, Xenopus tropicalis, Recombinant (His) | Xenopus tropicalis | E. coli | ||
RNA-binding protein that is involved in the post-transcriptional regulation of mRNAs. Plays a role in the regulation of mRNA stability, alternative splicing and translation. Binds to AU-rich element (ARE) sequences in the 3' untranslated region (3'UTR) of target mRNAs. Mainly plays a role in neuron-specific RNA processing.
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TMPH-03733 | Zaire ebolavirus (strain Kikwit-95) VP35 Protein (His & Myc) | ZEBOV | HEK293 Cells | ||
Plays an essential role in viral RNA synthesis and also a role in suppressing innate immune signaling. Acts as a polymerase cofactor in the RNA polymerase transcription and replication complexes. Serves as nucleoprotein/NP monomer chaperone prior to the formation of the large oligomeric RNA-bound complexes. Regulates RNA synthesis by modulating NP-RNA interactions and interacting with DYNLL1. VP35-NP interaction controls the switch between RNA-bound NP and free NP and thus the switch between genome replication and genome packaging into the nucleocapsid. Prevents establishment of cellular antiviral state, thereby suppressing host DC maturation. Acts by inhibiting host DDX58/RIG-I activation both by shielding dsRNA from detection and by preventing PRKRA binding to DDX58. Blocks virus-induced phosphorylation and activation of interferon regulatory factor 3/IRF3, a transcription factor critical for the induction of interferons alpha and beta. This blockage is produced through the interaction with and inhibition of host IKBKE and TBK1, producing a strong inhibition of the phosphorylation and activation of IRF3. Also inhibits the antiviral effect mediated by the host interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR. Increases PIAS1-mediated SUMOylation of IRF7, thereby repressing interferon transcription. Also acts as a suppressor of RNA silencing by interacting with host DICER1, TARBP2/TRBP and PRKRA/PACT. As a dimer, binds and sequesters dsRNA contributing to the inhibition of interferon production.
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TMPH-01799 | Nucleolar transcription factor 1 Protein, Human, Recombinant (E. coli, His & SUMO) | Human | E. coli | ||
Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element. Nucleolar transcription factor 1 Protein, Human, Recombinant (E. coli, His & SUMO) is expressed in E. coli expression system with N-6xHis-SUMO tag. The predicted molecular weight is 95.2 kDa and the accession number is P17480.
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TMPH-01798 | Nucleolar transcription factor 1 Protein, Human, Recombinant (Cell-Free, His & SUMO) | Human | E. coli | ||
Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element. Nucleolar transcription factor 1 Protein, Human, Recombinant (Cell-Free, His & SUMO) is expressed in E. coli expression system with N-10xHis-SUMO tag. The predicted molecular weight is 107.9 kDa and the accession number is P17480.
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TMPH-01247 | POLR3K Protein, Human, Recombinant (His) | Human | Baculovirus Insect Cells | ||
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
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TMPH-00741 | Rho Protein, E. coli, Recombinant (His) | E. coli | E. coli | ||
Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template. RNA-dependent NTPase which utilizes all four ribonucleoside triphosphates as substrates. Rho Protein, E. coli, Recombinant (His) is expressed in E. coli expression system with N-6xHis tag. The predicted molecular weight is 51.0 kDa and the accession number is P0AG30.
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TMPH-01246 | POLR3A Protein, Human, Recombinant (His) | Human | E. coli | ||
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
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TMPH-00251 | Bovine coronavirus (strain OK-0514) Nucleoprotein/NP Protein (His) | BCoV | E. coli | ||
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Bovine coronavirus (strain OK-0514) Nucleoprotein/NP Protein (His) is expressed in E. coli expression system with C-6xHis tag. The predicted molecular weight is 50.3 kDa and the accession number is P26020.
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TMPH-00138 | IBV (strain KB8523) Nucleoprotein/NP Protein (His) | IBV | HEK293 Cells | ||
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. IBV (strain KB8523) Nucleoprotein/NP Protein (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 47.2 kDa and the accession number is P12648.
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TMPH-01154 | Human coronavirus OC43 Nucleoprotein/NP Protein (His) | HCoV-OC43 | E. coli | ||
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Human coronavirus OC43 Nucleoprotein/NP Protein (His) is expressed in E. coli expression system with N-6xHis tag. The predicted molecular weight is 55.3 kDa and the accession number is P33469.
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