目录号 | 产品详情 | 靶点 | |
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T83957 | |||
CELT-211是一种强效且选择性的h5HT2B血清素受体荧光配体。它对h5HT2B显示出完全选择性,与h5HT2A、h5HT2C相比,其在放射配体结合测定中针对h5HT2B受体的Ki值为56.3 nM。激发和发射峰值(λ)分别为589和616 nm。这些波长与CoraFlor 1 TR-FRET供体一起使用作为TR-FRET测定中的受体染料是兼容的。 | |||
T83934 | |||
DMHBO+是一种阳离子荧光团。通过与辣椒适体结合激活其荧光(Kd = 12 nM),形成的Chili-DMHBO+复合体模仿红色荧光蛋白,适用于细胞中RNA的成像。它是理想的FRET供体,可与罗丹明染料Atto 590配合使用,在基于FRET的RNA分析系统中有应用。激发/发射峰值(λ) = 456/592 nm;量子产率 = 0.1;斯托克斯位移 = 136 nm。 | |||
T83820 | |||
TNP-GTP是一种荧光衍生物,源自蛋白质合成与糖异生的能量底物鸟苷三磷酸(GTP)。其在水中激发波长为410 nm时,发射最大波长为552 nm,而在40%和80%的N,N-二甲基甲酰胺中,其荧光强度增加,发射波长分别向544 nm和532 nm移动,这是因为N,N-二甲基甲酰胺的极性比水小。TNP-GTP是谷氨酸脱氢酶的抑制剂(Ki = 2.7 μM)。当与谷氨酸脱氢酶结合时,TNP-GTP的荧光强度增加,且发射波长从552 nm变为545 nm,此效果可以通过加入GTP来阻断。TNP-GTP还是嘌呤P2X2和P2X2/3受体的拮抗剂(IC50s分别为0.4和1.2 nM)。它还选择性抑制大鼠可溶性鸟苷酸环化酶(sGC; Ki = 11 nM)高于牛肝谷氨酸脱氢酶(GDH; Ki = 2.7 µM)以及钙调素依赖的B. pertussis腺苷酸环化酶(AC)毒素(Kis在存在锰或镁时分别为20和320 µM)。 | |||
T37218 | |||
N-methyl Mesoporphyrin IX 是广泛使用的 G-quadruplex DNA 特异性荧光结合剂,能有效监测 Aβ 纤颤。N-methyl Mesoporphyrin IX 对 G-四链体 DNA 敏感,对双链体、三链体和单链形式的 DNA 没有反应。N-methyl Mesoporphyrin IX 需通过与 Aβ 组装体堆叠才能发出强荧光。同时 N-methyl Mesoporphyrin IX 是一种原位抑制剂,是体外和细胞内 Aβ 淀粉样蛋白生成的非原位监测器。 | |||
T38071 | |||
The biology of highly reactive oxygen radical species is of great interest in many biomedical research disciplines, including neurodegeneration, aging, cancer, and infectious diseases.[1] There are a number of fluorescent reagents, such as 2,7-dichlorodihydrofluorescein (DCDHF), that can be used to detect free radicals, but they have significant limitations due to their facile oxidation by light and numerous non-radical oxidants such as hydrogen peroxide (H2O2). [2] HPF is a cell-permeable aromatic amino-fluorescein derivative that has little intrinsic fluorescence. [3] It undergoes oxidation only by highly reactive oxygen species (hROS) such as the hydroxyl radical, peroxynitrite, and hROS generated from a peroxidase/H2O2 system. It is inert to hypochlorite ion, nitric oxide, hydrogen peroxide (H2O2), superoxide, and other oxidants. Upon oxidation, HPF is converted to the highly fluorescent molecule fluorescein, with excitation/emission maxima of 490/515 nm, respectively, allowing the simple direct detection of highly reactive biological radicals. | |||
T37762 | |||
Fura-FF AM is a cell-permeable acetoxymethyl ester of the fluorescence calcium indicator fura-FF (potassium salt) . As fura-FF AM enters cells, it is hydrolyzed by intracellular esterases to produce fura-FF. Fura-FF is a difluorinated derivative of the calcium indicator fura-2 . Unlike, fura-2, fura-FF has negligible magnesium sensitivity, thus reducing interference from this cation. Fura-FF also has a higher calcium dissociation constant than fura-2 (Kd(calcium) = 6 and 0.14 μM, respectively). However, the spectral properties of fura-FF and fura-2 are similar with fura-FF displaying excitation/emission spectra of 365/514 nm in the absence of calcium, with a shift to 339/507 nm in the presence of a high calcium concentration. Low affinity calcium dyes, including fura-FF, are preferred for studying compartments with high concentrations of calcium, such as mitochondria, or in cell systems that have relatively low calcium buffering capacities, such as neuronal dendrites and spines. | |||
T35812 | |||
Sirtuins (SIRTs) represent a distinct class of trichostatin A-insensitive lysyl-deacetylases (class III HDACs). Human SIRT1 is the homolog of yeast silent information regulator 2 (Sir2) and has been shown to regulate the activity of the p53 tumor suppressor and inhibit apoptosis. Small molecule activators of SIRT1, such as resveratrol, extend lifespan in yeast and C. elegans in a manner that resembles caloric restriction. CAY10591 has been identified as an activator of the enzyme SIRT1. This compound increases fluorescence by 233% in a SIRT1 activity assay. [Activator activity was defined as the percentage of signal increase relative to signal window in the following formula: 100 x (Sample - Signallow)/(Signalhigh - Signallow)]. CAY10591 suppresses TNF-α in a dose-dependent manner. In THP-1 cells, TNF-α levels decreased from 325 pg/ml (control) to 104 and 53 pg/ml with 20 and 60 µM CAY10591, respectively. This activator also has a significant dose-dependent effect on fat mobilization in differentiated adipocytes, which would indicate the potential of SIRT1 activators for anti-obesity or anti-diabetic purposes. | |||
T80266 | Bombesin Receptor | ||
GB-6是一种短线性肽,专门针对胃泌素释放肽受体(GRPR)。该受体在胰腺癌细胞中高表达。由于GB-6具有肿瘤选择性和肿瘤特异性积累的特点,其结合近红外(NIR)荧光染料或99mTc标记后可作为提供高对比度的成像探针。此外,GB-6在体内具有良好稳定性,并在SW1990皮下异种移植模型中显示出5.2和6.3的高肿瘤/胰腺及肿瘤/肠道荧光信号比。因此,GB-6能迅速定位肿瘤并准确描绘其边界,展示出其在临床应用中的巨大潜力。 | |||
T37002 | |||
7-Fluoro-2,1,3-benzoxadiazole-4-sulfonate (SBD-F) is a thiol-reactive fluorogenic probe.1It has been used to quantify the levels of homocysteine, cysteine, and cysteamine in human plasma.2SBD-F displays excitation/emission maxima of 380/515 nm, respectively.1 1.Imai, K., Toyo’oka, T., and Watanabe, Y.A novel fluorogenic reagent for thiols: Ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonateAnal. Biochem.128(2)471-473(1983) 2.Ichinose, S., Nakamura, M., Maeda, M., et al.A validated HPLC-fluorescence method with a semi-micro column for routine determination of homocysteine, cysteine and cysteamine, and the relation between the thiol derivatives in normal human plasmaBiomed. Chromatogr.23(9)935-939(2009) | |||
T80242 | MMP | ||
TNO211为具备生物活性的肽,主要研究对象是基质金属蛋白酶(MMP),一类能够降解多种细胞外基质蛋白并调控诸多生物活性分子的肽链内切酶家族。MMP在细胞表面受体裂解、凋亡配体释放及趋化因子/细胞因子失活等过程中起作用,同时与细胞增殖、迁移(粘附/分散)、分化、血管生成、细胞凋亡及宿主防御等生物行为密切相关。TNO211可靶向MMP-2、8、12、13和14,包含MMP特定可裂解的Gly-Leu键,并具有EDANS/DABCYL荧光标记,其荧光检测在监测内皮细胞培养基和患者滑液中的MMP活性方面表现出高灵敏度。吸光度/电磁场测量参数为340/490 nm。 |
目录号 | 产品名/同用名 | 种属 | 表达系统 | ||
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TMPY-04510 | PSAP/Prosaposin Protein, Human, Recombinant (His) | Human | HEK293 Cells | ||
Prosaposin (PSAP) gene mutations, affecting saposin B (Sap-B) domain, cause a rare metachromatic leukodystrophy (MLD) variant in which arylsulfatase A (ARSA) activity is normal. The human prosaposin gene (PSAP) was previously localized to 10q21-->q22 by isotopic in situ hybridization using a human prosaposin cDNA as a probe. Using fluorescence in situ hybridization with a mouse genomic prosaposin fragment as probe, confirms the localization of PSAP and precisely maps it to band 10q22.1. PSAP/Prosaposin Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 57.9 kDa and the accession number is A0A024QZQ2.
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TMPY-00209 | PSAP/Prosaposin Protein, Rat, Recombinant (His) | Rat | HEK293 Cells | ||
Prosaposin (PSAP) gene mutations, affecting saposin B (Sap-B) domain, cause a rare metachromatic leukodystrophy (MLD) variant in which arylsulfatase A (ARSA) activity is normal. The human prosaposin gene (PSAP) was previously localized to 10q21-->q22 by isotopic in situ hybridization using a human prosaposin cDNA as a probe. Using fluorescence in situ hybridization with a mouse genomic prosaposin fragment as probe, confirms the localization of PSAP and precisely maps it to band 10q22.1. PSAP/Prosaposin Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 61 kDa and the accession number is A6K3X0.
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TMPY-04336 | Orange fluorescent protein Protein, Discosoma sp, Recombinant (His) | Discosoma sp | E. coli | ||
OFPSparkTM is a red (orange) fluorescent protein (excitation/emission maxima are 549 and 566 nm, respectively) derived from DsRed. Possessing high photostability and pH stability, OFPSparkTM is more than twice brighter than mOrange2. Fast OFPSparkTM maturation makes it detectable in mammalian cells as early as within 8 hrs after transfection. OFPSparkTM can be expressed and detected in a wide range of organisms. Mammalian cells transiently transfected with OFPSparkTM expression vectors produce bright fluorescence in 8 hrs after transfection. No cytotoxic effects or visible protein aggregation are observed. For its monomer structure, OFPSparkTM performs well in some fusions and protein labeling applications.
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TMPY-04268 | NUDC Protein, Human, Recombinant (His) | Human | HEK293 Cells | ||
NUDC (Nuclear Distribution C, Dynein Complex Regulator) is a Protein Coding gene. NUDC consists of at least 9 exons ranging from 66 bp to 266 bp in size and 8 introns from 92 bp to 2.0 kb in length, and the total genomic region spans about 8 kb. NUDC was mapped to 1p34-p35 by fluorescence in situ hybridization. This gene encodes a nuclear distribution protein that plays an essential role in mitosis and cytokinesis. Nuclear migration is essential for the growth, development, and cellular function of eukaryotes. NUDC protein plays an important role in nuclear migration. It is widely expressed in the testis, adrenal, and other tissues. NUDC protein is expressed highly in CNE-2 and HNE-2 cells. Anti-NUDC antibody could inhibit the growth of CNE-2 and HNE-2 cells.
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