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FGF-2 Protein, Human, Recombinant

产品编号 TMPY-00749FGF-2 Protein, Human, Recombinant

FGF-2 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 16.5 kDa and the accession number is P09038-4.

FGF-2 Protein, Human, Recombinant

FGF-2 Protein, Human, Recombinant

产品编号 TMPY-00749FGF-2 Protein, Human, Recombinant
FGF-2 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 16.5 kDa and the accession number is P09038-4.
规格价格库存数量
10 μg
¥ 560
现货
20 μg
¥ 780
5日内发货
50 μg
¥ 1,240
5日内发货
100 μg
¥ 1,750
5日内发货
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实验操作小课堂
FGF-2 Protein, Human, Recombinant产品问题解答
请问 FGF-2 Protein,Human, Recombinant 来源是什么?
重组人 FGF-2 蛋白是一种重要的生长因子,广泛应用于细胞生物学、组织工程和药物开发等多个领域。该产品通过基因工程技术在宿主细胞中表达并纯化得到。然而,在生产过程中使用了动物来源的物料,这意味着 FGF-2 Protein, Human, Recombinant 不属于 "Animal-Free" 类别。
常见问题解答
如何将冻干的重组蛋白配置为储备液?
在打开盖⼦之前,先使用 10000-12000 rpm 的转速进行离心,20-30 秒,将附着在管盖或管壁上的冻⼲粉收集到管底,以避免损失。大多数蛋白可通过加入无菌蒸馏水来重新溶解。如果需要除水以外的其他稀释剂,产品 COA 中会对其进行说明。一般储备液浓度建议不要低于 100 μg/mL,分装储存避免反复冻融。
为什么某些重组蛋白溶液中还需要添加载体蛋白?
载体蛋白通常被用于提高蛋白的稳定性,防止蛋白在冷冻或解冻过程中粘附在管壁上。塑料管对蛋白有一定的吸附能力,这可能导致蛋白和管壁之间难以分离,导致溶液中蛋白的实际浓度下降,从而影响其活性。为减少这种损失,在长期保存重组蛋白 产品之前,建议添加常用的载体蛋白溶液,例如0.1% BSA(牛血清白蛋白)、5% HSA(人血清白蛋白)、10% FBS(胎牛血清)或 5% 海藻糖等。如果重组蛋白的浓度较低,强烈建议添加适量的载体蛋白。
为什么重组蛋白的体积和质量与预期不符?
蛋白冻干粉由蛋白原液冻干而来,冻干粉质量不等同于蛋白质量。由于冻⼲产品的体积大⼩受多种因素影响,包括冻⼲前缓冲液的组分、盐离⼦浓度以及产品本身的浓度等。冻⼲粉可能看上去很多,也可能肉眼不易观察到,这些都是正常现象,请您放心使用。在收到产品后,照常复溶、使用或分装储存即可。
蛋白能保证活性吗?
我们有现货的产品,有活性数据的话会展示在官网生物活性那栏,没有数据的也不代表就一定没有活性,是我们还没有建立活性检测方法,没有进行测试,但是我们的蛋白都是优先按照有活性的方法去表达纯化的。
重组蛋白有吗?
您需要什么种属的蛋白呢,对表达系统和标签有要求吗,用于什么实验?
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产品信息

生物活性
1.iPSC-derived human vascular organoids (Day 7) were cultured with FGF2 , VEGFA , EGF . Red arrows represent vascular organoids. Image taken at 10x magnification.(Routinely tested) 2.Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.01-0.1 ng/mL.
FGF-2 Protein, Human, RecombinantFGF-2 Protein, Human, Recombinant
产品描述
FGF-2 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 16.5 kDa and the accession number is P09038-4.
种属
Human
表达系统
E. coli
标签Tag Free
蛋白编号P09038-4
别名
HBGF-2,fibroblast growth factor 2 (basic),FGFB,FGF-2,BFGF
蛋白构建
A DNA sequence encoding the mature form of human bFGF (NP_001997.5) (Pro143-Ser288) was expressed with an additional Met at the N-terminus. Predicted N terminal: Met
蛋白纯度
≥ 95 % as determined by SDS-PAGE
FGF-2 Protein, Human, Recombinant
分子量16.5 kDa (predicted)
内毒素< 10 EU/mg of the protein.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法
Reconstituted with sterile deionized water to 0.15 mg/mL.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
Basic fibroblast growth factor (bFGF), also known as FGF2, is a member of the fibroblast growth factor (FGF) family. It is a highly specific chemotactic and mitogenic factor for many cell types, appears to be involved in remodeling damaged tissue, such as ulcer healing, vascular repair, traumatic brain injury (TBI). bFGF is a critical component of human embryonic stem cell culture medium. In addition, bFGF protein is a heparin-binding cationic protein involved in a variety of pathological conditions including angiogenesis and solid tumour growth. Thus, bFGF is regarded as a target for cancers chemopreventive and therapeutic strategies.bFGF/FGF2 Protein & Antibody Products

参考文献

SCI 文献

计算器

  • 复溶 计算器
  • 重组蛋白稀释 计算器
  • 比活力 计算器

技术支持

请阅读 重组蛋白用户指南 了解更多具体信息.

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