FGF-2 Protein, Mouse, Recombinant

FGF basic is one of 22 mitogenic proteins of the FGF family, which show 35-60% amino acid conservation. Unlike other FGFs, FGF acidic and basic lack signal peptides and are secreted by an alternate pathway. The 17 kDa mouse sequence has 98% aa identity with rat, and 95% identity with human, bovine, and sheep FGF basic. Binding of FGF to heparin or cell surface HSPG is necessary for binding, dimerization and activation of tyrosine kinase FGF receptors. FGF basic binds other proteins, polysaccharides and lipids with lower affinity. Expression of FGF basic is nearly ubiquitous but disruption of the mouse FGF basic gene gives a relatively mild phenotype, suggesting compensation by other FGF family members. FGF basic modulates such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, neuronal function and neural degeneration. Transgenic overexpression of FGF basic results in excessive proliferation and angiogenesis is reminiscent of a variety of pathological conditions.

规格	价格/CNY		数量
10 μg	￥ 380	现货
50 μg	￥ 1,090	5日内发货
500 μg	￥ 4,860	5日内发货
1 mg	￥ 6,950	5日内发货

规格

价格/CNY

货期

数量

10 μg

￥ 380

现货

50 μg

￥ 1,090

5日内发货

500 μg

￥ 4,860

5日内发货

1 mg

￥ 6,950

5日内发货

生物活性

Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 0.3-1.8 ng/ml. (QC verified)

TargetMol Bioactive

产品描述

种属	Mouse
表达系统	E. coli
标签	None
蛋白编号	P15655
别名	Fibroblast Growth Factor 2, bFGF, HBGF-2, Basic Fibroblast Growth Factor, Fgf-2, Fgf2, Heparin-Binding Growth Factor 2
氨基酸序列	Met1-Ser154
蛋白构建	Recombinant Mouse Fibroblast Growth Factor 2/Fibroblast Growth Factor Basic is produced by our E.coli expression system and the target gene encoding Met1-Ser154 is expressed.
蛋白纯度	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
分子量	16 KDa, reducing conditions

内毒素	Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.
缓冲液	Lyophilized from a 0.2 μm filtered solution of 20mM PB, 400mM NaCl, 0.02% Tween 80, 4.0% Sucrose, 4.0% Manntiol, pH 7.0.
复溶方法	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
存储	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
运输方式	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
研究背景	FGF basic is one of 22 mitogenic proteins of the FGF family, which show 35-60% amino acid conservation. Unlike other FGFs, FGF acidic and basic lack signal peptides and are secreted by an alternate pathway. The 17 kDa mouse sequence has 98% aa identity with rat, and 95% identity with human, bovine, and sheep FGF basic. Binding of FGF to heparin or cell surface HSPG is necessary for binding, dimerization and activation of tyrosine kinase FGF receptors. FGF basic binds other proteins, polysaccharides and lipids with lower affinity. Expression of FGF basic is nearly ubiquitous but disruption of the mouse FGF basic gene gives a relatively mild phenotype, suggesting compensation by other FGF family members. FGF basic modulates such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, neuronal function and neural degeneration. Transgenic overexpression of FGF basic results in excessive proliferation and angiogenesis is reminiscent of a variety of pathological conditions.

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FGF-2 Protein, Mouse, Recombinant

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