FGF-2 Protein, Human, Recombinant (K128N) is expressed in E. coli expression system. The predicted molecular weight is 17 KDa and the accession number is P09038-2.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
10 μg | ¥ 540 | 5日内发货 | ||
50 μg | ¥ 1,170 | 5日内发货 | ||
500 μg | ¥ 5,250 | 5日内发货 | ||
1 mg | ¥ 7,510 | 5日内发货 |
产品描述 | FGF-2 Protein, Human, Recombinant (K128N) is expressed in E. coli expression system. The predicted molecular weight is 17 KDa and the accession number is P09038-2. |
种属 | Human |
表达系统 | E. coli |
标签 | None |
蛋白编号 | P09038-2 |
别名 | FGF2, bFGF, FGFB, Heparin-binding growth factor 2, HBGF-2, Fibroblast growth factor 2, Basic fibroblast growth factor, FGF-2 |
氨基酸序列 | Met1-Ser155 |
蛋白构建 | Recombinant Human Thermostable Fibroblast Growth Factor 2 is produced by our E.coli expression system and the target gene encoding Met1-Ser155 is expressed. |
蛋白纯度 | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
分子量 | 17 KDa, reducing conditions |
内毒素 | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
缓冲液 | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 200mM NaCl, pH 7.5. |
存储 |
Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
运输方式 |
The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below. |
研究背景 | Fibroblast growth factors (FGF) are a family of heparin-binding secreted proteins that stimulate cell proliferation and differentiation in a wide variety of tissues. FGFs play important roles in diverse biological functions both in vivo and in vitro, including mitogenesis, cellular migration, differentiation, angiogenesis, and wound healing. Human embryonic stem cell (hESC) cultures require FGF basic (also known as FGF-2 or bFGF) in cell culture media to remain in an undifferentiated and pluripotent state. Thermostable FGF basic was engineered for enhanced stability in culture media, without modification of its biological function. FGF basic is a required component of stem cell culture media for maintaining cells in an undifferentiated state. Because FGF basic is unstable, daily media changes are needed. The thermostable FGF basic that supports a 2-day media change schedule, so no media changes are required over a weekend. This thermostable FGF basic was more stable than FGF basic in biochemical studies, and maintained cell growth, pluripotency and differentiation potential with a 2-day feeding schedule. |
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FGF-2 Protein, Human, Recombinant (K128N) FGF2 bFGF FGFB HBGF2 Heparin-binding growth factor 2 FGF 2 HBGF-2 Fibroblast growth factor 2 HBGF 2 Basic fibroblast growth factor FGF-2 recombinant recombinant-proteins proteins protein