Powder: -20°C for 3 years | In solvent: -80°C for 1 year
D159687 是一种PDE4D 选择性抑制剂。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 218 | 现货 | ||
5 mg | ¥ 495 | 现货 | ||
10 mg | ¥ 792 | 现货 | ||
25 mg | ¥ 1,670 | 现货 | ||
50 mg | ¥ 2,670 | 现货 | ||
100 mg | ¥ 3,990 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 547 | 现货 |
产品描述 | D159687 is a selective PDE4D inhibitor,had a procognitive profile as it improved memory in the novel object recognition test but had no antidepressant or anxiolytic benefit. |
体外活性 | D159687 induced a transient increase in CREB phosphorylation which peaked at 6 hrs after treatment ( F (3, 20) = 3.731, P = 0.0425). CREB phosphorylation was optimal at 1 μM ( F (3, 20) = 4.194, P = 0.0302). |
细胞实验 | cAMP concentrations were measured using the enzyme-linked immunosorbent assay.?On the day of treatment, the attached HT-22 cells were washed with warm phosphate buffer saline and then incubated for 10 min with PBS supplemented with various concentrations of D159687 and further for 10 min with 10 nM isoproterenol in the system52.?After incubation, PBS containing the drugs was removed and the plates were let dry roughly before 200 μl HCl (0.1 M) was added to each well to lyse the cells.?Cell lysates were collected into Eppendorf tubes and centrifuged at 800 g for 15 min. Supernatants were collected for immediate assay or stored frozen for assay later using the cAMP complete ELISA kit according to the assay protocol. |
别名 | D 159687 |
分子量 | 366.84 |
分子式 | C21H19ClN2O2 |
CAS No. | 1155877-97-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 150 mg/mL (408.90 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.726 mL | 13.6299 mL | 27.2598 mL | 68.1496 mL |
5 mM | 0.5452 mL | 2.726 mL | 5.452 mL | 13.6299 mL | |
10 mM | 0.2726 mL | 1.363 mL | 2.726 mL | 6.815 mL | |
20 mM | 0.1363 mL | 0.6815 mL | 1.363 mL | 3.4075 mL | |
50 mM | 0.0545 mL | 0.2726 mL | 0.5452 mL | 1.363 mL | |
100 mM | 0.0273 mL | 0.1363 mL | 0.2726 mL | 0.6815 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
D159687 1155877-97-6 Metabolism PDE Inhibitor inhibit D-159687 D 159687 Phosphodiesterase (PDE) inhibitor