TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 2,960 | 5日内发货 | ||
500 μg | ¥ 11,800 | 5日内发货 | ||
1 mg | ¥ 19,700 | 5日内发货 |
生物活性 | 1. Immobilized Human TNFSF15 Trimer, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 10.2ng/ml determined by ELISA. 2. Mouse DR3, His Tag immobilized on CM5 Chip can bind Human TNFSF15 (Trimer) , His Tag with an affinity constant of 3.06 nM as determined in SPR assay. |
产品描述 | TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2. |
种属 | Human |
表达系统 | HEK293 |
标签 | N-His-Flag |
蛋白编号 | O95150-1 |
别名 | TL1A, TL1, TNFSF15, VEGI, VEGI192A, VEGI-251 |
蛋白构建 | Recombinant Human TNFSF15 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus. It contains Asp91-Leu251.[Accession |O95150-1] |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | The protein has a predicted MW of 58.2 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result. |
内毒素 | Less than 1EU per μg by the LAL method. |
缓冲液 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
复溶方法 | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
存储 |
-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
运输方式 |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
研究背景 | TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2. |
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TNFSF15 Trimer Protein, Human, Recombinant (His & Flag) VEGI 251 TNFSF 15 TL1A TL1 TNFSF15 VEGI251 TNFSF-15 VEGI VEGI192A TL-1 VEGI-251 TL 1 recombinant recombinant-proteins proteins protein