TNF RII Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-Fc tag. The predicted molecular weight is 60-90 KDa and the accession number is P20333.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
10 μg | ¥ 740 | 5日内发货 | ||
50 μg | ¥ 1,970 | 5日内发货 | ||
500 μg | ¥ 12,100 | 5日内发货 | ||
1 mg | ¥ 17,400 | 5日内发货 |
生物活性 | 1. Loaded Recombinant Human TNF RII (C-Fc) on Pro-A Biosensor, can bind Recombinant Human TNF alpha with an affinity constant of 25.2 pM as determined in BLI assay. (Regularly tested) 2. Measured by its ability to induce NF-κB reporter activity in HEK293 human embryonic kidney cells. Recombinant Human TNF RII inhibits a constant dose of 0.5ng/mL of Recombinant TNF alpha. The IC50 for this effect is 2.5 ng/mL. (Regularly tested) 3. Immobilized Recombinant Human TNF alpha at 5μg/ml (100 μl/well) can bind Recombinant Human TNF RII (C-Fc) . The ED50 of Recombinant Human TNF RII (C-Fc) is 15.35 ng/ml. (Regularly tested) |
产品描述 | TNF RII Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-Fc tag. The predicted molecular weight is 60-90 KDa and the accession number is P20333. |
种属 | Human |
表达系统 | Human Cells |
标签 | C-Fc |
蛋白编号 | P20333 |
别名 | TNF-R2, Tumor necrosis factor receptor superfamily member 1B, TNF-RII, TNFRSF1B, Tumor necrosis factor receptor 2, p75, p80 TNF-α receptor, p80 TNF-alpha receptor, CD120b, Tumor necrosis factor receptor type II |
氨基酸序列 | Leu23-Asp257 |
蛋白构建 | Recombinant Human Tumor Necrosis Factor Receptor II is produced by our Mammalian expression system and the target gene encoding Leu23-Asp257 is expressed with a human IgG1 Fc tag at the C-terminus. |
蛋白纯度 | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
分子量 | 60-90 KDa, reducing conditions |
内毒素 | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
缓冲液 | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
复溶方法 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
存储 |
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
运输方式 |
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. |
研究背景 | Tumor necrosis factor receptor superfamily member 1B (TNFRSF1B) is a member of the tumor necrosis factor receptor superfamily. Human TNF RII contains four cysteinerich repeats in its ECD, which shares 58% and 56% amino acid sequence identity with the mouse and rat orthologs, respectively.TNF RII is expressed predominantly on cells of the hematopoietic lineage, such as T and natural killer cells, as well as on endothelial cells, microglia, astrocytes,neurons, oligodendrocytes, cardiac myocytes, thymocytes, and mesenchymal stem cells.TNF RII binds to the membranebound forms of TNFα and Lymphotoxinα/TNFβ;soluble TNF is thought to signal predominately through TNF RI.Soluble TNF RII is believed to inhibit TNF biological activity by binding TNF thereby preventing it from activating membrane TNF receptors. |
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TNF RII Protein, Human, Recombinant (hFc) TNF-R2 Tumor necrosis factor receptor superfamily member 1B TNF-RII TNFRSF1B Tumor necrosis factor receptor 2 p75 p80 TNF-α receptor p80 TNF-alpha receptor p80 TNF-a receptor CD120b Tumor necrosis factor receptor type II recombinant recombinant-proteins proteins protein