Protein Kinase D2/PRKD2 Protein, Human, Recombinant (His & GST)

Serine/threonine-protein kinase D2, also known as PRKD2 and PKD2, is a cytoplasm and membrane protein that belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and PKD subfamily. PRKD2 / PKD2 is widely expressed. It contains one PH domain, two phorbol-ester/DAG-type zinc fingers and one protein kinase domain. PRKD2 / PKD2 is activated by DAG and phorbol esters. Phorbol-ester/DAG-type domains bind DAG, mediating translocation to membranes. Autophosphorylation of Ser-71 and phosphorylation of Ser-76 by PKC relieves auto-inhibition by the PH domain. PRKD2 / PKD2 converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.

规格	价格/CNY		货期	数量
50 μg	￥ 3,170	5日内发货

规格

价格/CNY

货期

数量

50 μg

￥ 3,170

5日内发货

生物活性

The specific activity was determined to be ＞30 nmol/min/mg using synthetic CREBtide peptide (KRREILSRRPSYR) as substrate.

产品描述

种属	Human
表达系统	Baculovirus-Insect Cells
标签	His,GST
蛋白编号	Q9BZL6-1
别名	protein kinase D2, nPKC-D2, PKD2, HSPC187
蛋白构建	A DNA sequence encoding the amino acid sequence (Met 1-Leu 878) of human PRKD2 (NP_057541.2) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
蛋白纯度	> 82 % as determined by SDS-PAGE
分子量	Approxiamtely 124 kDa

内毒素	< 1.0 EU per μg of the protein as determined by the LAL method
缓冲液	Supplied as sterile 50mM Tris, 500mM NaCl, 0. 5mM PMSF, 10% glycerol, pH 8.0. Pleasecon tact usfor any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.
复溶方法	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
存储	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
运输方式	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.
研究背景	Serine/threonine-protein kinase D2, also known as PRKD2 and PKD2, is a cytoplasm and membrane protein that belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and PKD subfamily. PRKD2 / PKD2 is widely expressed. It contains one PH domain, two phorbol-ester/DAG-type zinc fingers and one protein kinase domain. PRKD2 / PKD2 is activated by DAG and phorbol esters. Phorbol-ester/DAG-type domains bind DAG, mediating translocation to membranes. Autophosphorylation of Ser-71 and phosphorylation of Ser-76 by PKC relieves auto-inhibition by the PH domain. PRKD2 / PKD2 converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.