Noggin/NOG Protein, Mouse, Recombinant (His)

Noggin is a secreted homodimeric glycoprotein that is an antagonist of bone morphogenetic proteins (BMPs). Mouse Noggin cDNA encodes a 232 amino acid (aa) residue precursor protein with 19 aa residue putative signal peptide that is cleaved to generate the 213 aa residue mature protein which is secreted as a homodimeric glycoprotein. Secreted Noggin probably remains close to the cell surface due to its binding of heparin-containing proteoglycans. Noggin binds some BMPs such as BMP4 with high affinity and others such as BMP7 with lower affinity. It antagonizes BMP bioactivities by blocking epitopes on BMPs that are needed for binding to both type I and type II receptors. Noggin is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle and skin. During culture of human embryonic stem cells (hESC) or neural stem cells under certain conditions, addition of Noggin to antagonize BMP activity may allow stem cells to proliferate while maintaining their undifferentiated state, or alternatively, to differentiate into dopaminergic neurons.

规格	价格/CNY		数量
10 μg	￥ 700	现货
50 μg	￥ 1,970	5日内发货
500 μg	￥ 9,960	5日内发货
1 mg	￥ 14,200	5日内发货

规格

价格/CNY

货期

数量

10 μg

￥ 700

现货

50 μg

￥ 1,970

5日内发货

500 μg

￥ 9,960

5日内发货

1 mg

￥ 14,200

5日内发货

生物活性

Mouse intestinal organoids were cultured with EGF , Wnt3a , Noggin and R-spondin 1 . After five days of culture, the organoids showed good morphology and germination rate.

TargetMol Bioactive

产品描述

种属	Mouse
表达系统	Human Cells
标签	C-6His
蛋白编号	P97466
别名	Nog, Noggin
氨基酸序列	Gln28-Cys232
蛋白构建	Recombinant Mouse Noggin is produced by our Mammalian expression system and the target gene encoding Gln28-Cys232 is expressed with a 6His tag at the C-terminus.
蛋白纯度	Greater than 95% as determined by reducing SDS-PAGE.; Greater than 95% as determined by SEC-HPLC.
分子量	30 KDa, reducing conditions

内毒素	Less than 1 EU/μg as determined by LAL test.
缓冲液	Lyophilized from a 0.2 μm filtered solution of PBS, 5mM EDTA, 5% Trehalose, pH 7.4.
复溶方法	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
存储	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
运输方式	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
研究背景	Noggin is a secreted homodimeric glycoprotein that is an antagonist of bone morphogenetic proteins (BMPs). Mouse Noggin cDNA encodes a 232 amino acid (aa) residue precursor protein with 19 aa residue putative signal peptide that is cleaved to generate the 213 aa residue mature protein which is secreted as a homodimeric glycoprotein. Secreted Noggin probably remains close to the cell surface due to its binding of heparin-containing proteoglycans. Noggin binds some BMPs such as BMP4 with high affinity and others such as BMP7 with lower affinity. It antagonizes BMP bioactivities by blocking epitopes on BMPs that are needed for binding to both type I and type II receptors. Noggin is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle and skin. During culture of human embryonic stem cells (hESC) or neural stem cells under certain conditions, addition of Noggin to antagonize BMP activity may allow stem cells to proliferate while maintaining their undifferentiated state, or alternatively, to differentiate into dopaminergic neurons.

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比活力计算器

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

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Noggin/NOG Protein, Mouse, Recombinant (His)

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