LAIR1 Protein, Rhesus macaque, Recombinant (His)

Leukocyte-Associated Immunoglobulin-Like Receptor 1 (LAIR1) is a single-pass type I membrane protein. LAIR1 expressed on the majority of peripheral mononuclear cells, including natural killer (NK) cells, T-cells, B-cells, monocytes, and dendritic cells, highly in naive T-cells and B-cells. As an inhibitory receptor, LAIR1 plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. LAIR1 plays inhibitory role independently of SH2-containing phosphatases and modulates cytokine production in CD4+ T-cells. It down-regulates IL2 and IFNG production while inducing secretion of transforming growth factor beta, also down-regulates IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion. LAIR1 inhibits the differentiation of peripheral blood precursors towards dendritic cells. It also restrains proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells.

规格	价格/CNY		数量
10 μg	￥ 550	5日内发货
50 μg	￥ 1,670	5日内发货
500 μg	￥ 13,700	5日内发货
1 mg	￥ 20,500	5日内发货

规格

价格/CNY

货期

数量

10 μg

￥ 550

5日内发货

50 μg

￥ 1,670

5日内发货

500 μg

￥ 13,700

5日内发货

1 mg

￥ 20,500

5日内发货

产品描述

种属	Rhesus Macaque
表达系统	Human Cells
标签	C-6His
蛋白编号	A0A0U3SBU1
别名	LAIR1, CD305, hLAIR1, LAIR-1, Leukocyte-Associated Immunoglobulin-Like Receptor 1
氨基酸序列	Gln22-Try165
蛋白构建	Recombinant Rhesus Macaque Leukocyte-Associated Immunoglobulin-Like Receptor 1 is produced by our Mammalian expression system and the target gene encoding Gln22-Try165 is expressed with a 6His tag at the C-terminus.
蛋白纯度	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
分子量	25-33 KDa, reducing conditions

内毒素	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
缓冲液	Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
复溶方法	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
存储	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
运输方式	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
研究背景	Leukocyte-Associated Immunoglobulin-Like Receptor 1 (LAIR1) is a single-pass type I membrane protein. LAIR1 expressed on the majority of peripheral mononuclear cells, including natural killer (NK) cells, T-cells, B-cells, monocytes, and dendritic cells, highly in naive T-cells and B-cells. As an inhibitory receptor, LAIR1 plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. LAIR1 plays inhibitory role independently of SH2-containing phosphatases and modulates cytokine production in CD4+ T-cells. It down-regulates IL2 and IFNG production while inducing secretion of transforming growth factor beta, also down-regulates IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion. LAIR1 inhibits the differentiation of peripheral blood precursors towards dendritic cells. It also restrains proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells.

计算器

复溶计算器

重组蛋白稀释计算器

比活力计算器

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

bottom

LAIR1 Protein, Rhesus macaque, Recombinant (His)

计算器

技术支持

Keywords