GPHA2 is a member of the glycoprotein hormones subunit alpha family. Glycoprotein hormones consist of two subunits, the common alpha- and specific beta-subunits, which associate noncovalently to form a heterodimer. The alpha-subunit combines with four distinct beta-subunits giving rise to four biologically active hormones in the human: FSH, LH, TSH, and CG. GPHA2 and glycoprotein hormone beta 5 (GPHB5) can form a noncovalent heterodimer. GPHA2 can be detected in a variety of tissues. Recombinant A2/B5 heterodimeric glycoproteins activate human TSH receptors, but not LH and FSH receptors, and shows high affinity to TSH receptors in a radioligand receptor assay. The heterodimer also stimulates cAMP production and thymidine incorporation by cultured thyroid cells and increases serum thyroxine levels in TSH-suppressed rats in vivo. This new heterodimeric glycoprotein hormone was named Thyrostimulin based on its thyroid-stimulating activity. The expression of Thyrostimulin in the anterior pituitary known to express TSH receptors suggested a paracrine mechanism.
生物活性 | Testing in progress |
产品描述 | GPHA2 is a member of the glycoprotein hormones subunit alpha family. Glycoprotein hormones consist of two subunits, the common alpha- and specific beta-subunits, which associate noncovalently to form a heterodimer. The alpha-subunit combines with four distinct beta-subunits giving rise to four biologically active hormones in the human: FSH, LH, TSH, and CG. GPHA2 and glycoprotein hormone beta 5 (GPHB5) can form a noncovalent heterodimer. GPHA2 can be detected in a variety of tissues. Recombinant A2/B5 heterodimeric glycoproteins activate human TSH receptors, but not LH and FSH receptors, and shows high affinity to TSH receptors in a radioligand receptor assay. The heterodimer also stimulates cAMP production and thymidine incorporation by cultured thyroid cells and increases serum thyroxine levels in TSH-suppressed rats in vivo. This new heterodimeric glycoprotein hormone was named Thyrostimulin based on its thyroid-stimulating activity. The expression of Thyrostimulin in the anterior pituitary known to express TSH receptors suggested a paracrine mechanism. |
种属 | Human |
表达系统 | HEK293 |
标签 | His |
蛋白编号 | Q96T91 |
别名 | GPA2, glycoprotein hormone α 2, A2, ZSIG51, glycoprotein hormone alpha 2 |
蛋白构建 | A DNA sequence encoding the human GPHA2 (Q96T91) (Met1-Tyr129) was expressed with a polyhistidine tag at the C-terminus. |
蛋白纯度 | > 90 % as determined by SDS-PAGE |
分子量 | Approxiamtely 13.1 kDa |
内毒素 | < 1.0 EU per μg of the protein as determined by the LAL method |
缓冲液 | Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
复溶方法 | A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information. |
存储 |
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
运输方式 |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
研究背景 | GPHA2 is a member of the glycoprotein hormones subunit alpha family. Glycoprotein hormones consist of two subunits, the common alpha- and specific beta-subunits, which associate noncovalently to form a heterodimer. The alpha-subunit combines with four distinct beta-subunits giving rise to four biologically active hormones in the human: FSH, LH, TSH, and CG. GPHA2 and glycoprotein hormone beta 5 (GPHB5) can form a noncovalent heterodimer. GPHA2 can be detected in a variety of tissues. Recombinant A2/B5 heterodimeric glycoproteins activate human TSH receptors, but not LH and FSH receptors, and shows high affinity to TSH receptors in a radioligand receptor assay. The heterodimer also stimulates cAMP production and thymidine incorporation by cultured thyroid cells and increases serum thyroxine levels in TSH-suppressed rats in vivo. This new heterodimeric glycoprotein hormone was named Thyrostimulin based on its thyroid-stimulating activity. The expression of Thyrostimulin in the anterior pituitary known to express TSH receptors suggested a paracrine mechanism. |
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GPHA2 Protein, Human, Recombinant (His) GPA-2 GPA2 A 2 glycoprotein hormone α 2 A2 ZSIG51 ZSIG-51 glycoprotein hormone a 2 A-2 glycoprotein hormone alpha 2 ZSIG 51 GPA 2 recombinant recombinant-proteins proteins protein