Frizzled-8 Protein, Human, Recombinant (His)

Frizzled-8 is one of at least ten seven-transmembrane (7TM) glycoproteins of the Frizzled family of Wnt receptors. Frizzled proteins are thought to be G-protein-coupled. Wnt engagement, with low density lipoprotein receptor-related proteins LRP-5 or LRP-6 acting as co-receptors, stabilizes beta -catenin and promotes gene transcription that is important in development and tissue maintenance. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. These ligands bind the extracellular CRD of Frizzled-8, blocking Wnt binding. The recombinant Frizzled-8 CRD has also been used to block Wnt signaling and inhibit growth of teratocarcinomas.

规格	价格/CNY		数量
10 μg	￥ 910	5日内发货
50 μg	￥ 2,760	5日内发货
500 μg	￥ 15,600	5日内发货
1 mg	￥ 23,400	5日内发货

规格

价格/CNY

货期

数量

10 μg

￥ 910

5日内发货

50 μg

￥ 2,760

5日内发货

500 μg

￥ 15,600

5日内发货

1 mg

￥ 23,400

5日内发货

产品描述

种属	Human
表达系统	Human Cells
标签	C-6His
蛋白编号	Q9H461
别名	FZD8, hFZ8, frizzled family receptor 8, frizzled 8, FZ-8, Frizzled8, Frizzled-8
氨基酸序列	Ala28-Pro172
蛋白构建	Recombinant Human Frizzled-8 is produced by our Mammalian expression system and the target gene encoding Ala28-Pro172 is expressed with a 6His tag at the C-terminus.
蛋白纯度	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
分子量	22-32 KDa, reducing conditions

内毒素	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
缓冲液	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
复溶方法	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
存储	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
运输方式	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
研究背景	Frizzled-8 is one of at least ten seven-transmembrane (7TM) glycoproteins of the Frizzled family of Wnt receptors. Frizzled proteins are thought to be G-protein-coupled. Wnt engagement, with low density lipoprotein receptor-related proteins LRP-5 or LRP-6 acting as co-receptors, stabilizes beta -catenin and promotes gene transcription that is important in development and tissue maintenance. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. These ligands bind the extracellular CRD of Frizzled-8, blocking Wnt binding. The recombinant Frizzled-8 CRD has also been used to block Wnt signaling and inhibit growth of teratocarcinomas.

计算器

复溶计算器

重组蛋白稀释计算器

比活力计算器

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

bottom

Frizzled-8 Protein, Human, Recombinant (His)

计算器

技术支持

Keywords