CD40 Ligand Protein, Human, Recombinant

CD40 Ligand (CD40LG) is a type II transmembrane glycoprotein that belongs to the TNF superfamily. Like other TNF superfamily members, CD40LG exists as a trimer in membrane bound and soluble form, both of which are bioactive. CD40LG is a ligand for CD40; its ligation also initiates signal transduction in CD40LG expressing cells. CD40LG is a differentiation antigen that is expressed on the surface of T-cells. It stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40LG has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2.

规格	价格/CNY		数量
10 μg	￥ 380	5日内发货
50 μg	￥ 1,090	5日内发货
500 μg	￥ 4,860	5日内发货
1 mg	￥ 6,950	5日内发货

规格

价格/CNY

货期

数量

10 μg

￥ 380

5日内发货

50 μg

￥ 1,090

5日内发货

500 μg

￥ 4,860

5日内发货

1 mg

￥ 6,950

5日内发货

生物活性

1. Loaded Human CD40-His on HIS1K Biosensor, can bind Human CD40L with an affinity constant of 2.43 nM as determined in BLI assay. (Regularly tested) 2. Immobilized Human CD40-Fc at 5μg/ml (100 μl/well) can bind Human CD40L . The ED50 of Human CD40L is 6.05 ng/ml. (Regularly tested)

产品描述

种属	Human
表达系统	E. coli
标签	None
蛋白编号	P29965
别名	CD40LG, CD40 Ligand, TRAP, TNF-Related Activation Protein, TNFSF5, Tumor Necrosis Factor Ligand Superfamily Member 5, T-Cell Antigen Gp39, CD40-L, CD154, CD40L
氨基酸序列	Met113-Leu261
蛋白构建	Recombinant Human CD40 Ligand is produced by our E.coli expression system and the target gene encoding Met113-Leu261 is expressed.
蛋白纯度	Greater than 95% as determined by reducing SDS-PAGE. Greater than 95% as determined by SEC-HPLC.
分子量	15 KDa, reducing conditions

内毒素	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
缓冲液	Lyophilized from a 0.2 μm filtered solution of 20mM PB, 200mM NaCl, 0.1mM EDTA, pH 7.0.
复溶方法	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
存储	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
运输方式	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
研究背景	CD40 Ligand (CD40LG) is a type II transmembrane glycoprotein that belongs to the TNF superfamily. Like other TNF superfamily members, CD40LG exists as a trimer in membrane bound and soluble form, both of which are bioactive. CD40LG is a ligand for CD40; its ligation also initiates signal transduction in CD40LG expressing cells. CD40LG is a differentiation antigen that is expressed on the surface of T-cells. It stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40LG has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2.

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CD40 Ligand Protein, Human, Recombinant

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