APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily. It is synthesized as a 32 kDa proprotein which is cleaved by furin in the Golgi to release the active 17 kDa soluble molecule. Secreted human APRIL, which consists almost entirely of a single TNF homology domain, shares 85% amino acid sequence identity with mouse and rat APRIL. Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R. APRIL binds to heparan sulfate proteoglycans (HSPGs) independently of its binding to TACI and BCMA. APRIL can form bioactive heterotrimers with BAFF, and these circulate in the serum of patients with rheumatic immune disorders. APRIL enhances the proliferation and survival of plasma cells and also promotes T cell-dependent humoral responses. APRIL levels are elevated in the serum during coronary artery disease, and it is also elevated in many cancers primarily due to expression by tumor-infiltratin neutrophils.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
10 μg | ¥ 820 | 现货 | ||
50 μg | ¥ 1,770 | 5日内发货 | ||
500 μg | ¥ 14,600 | 5日内发货 | ||
1 mg | ¥ 20,700 | 5日内发货 |
生物活性 |
1. Loaded Cynomolgus BCMA-Fc on Protein A Biosensor, can bind Human APRIL-His with an affinity constant of 0.005 nM as determined in BLI assay. (Regularly tested)
2. Immobilized Human APRIL -Flag-His at 1μg/ml (100 μl/well) can bind Cynomolgus BCMA-Fc. The ED50 of Cynomolgus BCMA-Fc is 4.23 ng/ml. (Regularly tested);
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产品描述 | APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily. It is synthesized as a 32 kDa proprotein which is cleaved by furin in the Golgi to release the active 17 kDa soluble molecule. Secreted human APRIL, which consists almost entirely of a single TNF homology domain, shares 85% amino acid sequence identity with mouse and rat APRIL. Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R. APRIL binds to heparan sulfate proteoglycans (HSPGs) independently of its binding to TACI and BCMA. APRIL can form bioactive heterotrimers with BAFF, and these circulate in the serum of patients with rheumatic immune disorders. APRIL enhances the proliferation and survival of plasma cells and also promotes T cell-dependent humoral responses. APRIL levels are elevated in the serum during coronary artery disease, and it is also elevated in many cancers primarily due to expression by tumor-infiltratin neutrophils. |
种属 | Human |
表达系统 | Human Cells |
标签 | N-Flag-His |
蛋白编号 | O75888 |
别名 | TRDL-1, TNF- and APOL-related leukocyte expressed ligand 2, Tumor necrosis factor ligand superfamily member 13, A proliferation-inducing ligand, TNF-related death ligand 1, TNFSF13, CD256, TALL-2, APRIL |
氨基酸序列 | Lys112-Leu250 |
蛋白构建 | Recombinant Human A proliferation-inducing Ligand is produced by our Mammalian expression system and the target gene encoding Lys112-Leu250 is expressed with a His, Flag tag at the N-terminus. |
蛋白纯度 |
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
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分子量 | 60 KDa, reducing conditions |
内毒素 | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
缓冲液 | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 1M NaCl, pH8.3. |
复溶方法 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
存储 |
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
运输方式 |
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. |
研究背景 | APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily. It is synthesized as a 32 kDa proprotein which is cleaved by furin in the Golgi to release the active 17 kDa soluble molecule. Secreted human APRIL, which consists almost entirely of a single TNF homology domain, shares 85% amino acid sequence identity with mouse and rat APRIL. Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R. APRIL binds to heparan sulfate proteoglycans (HSPGs) independently of its binding to TACI and BCMA. APRIL can form bioactive heterotrimers with BAFF, and these circulate in the serum of patients with rheumatic immune disorders. APRIL enhances the proliferation and survival of plasma cells and also promotes T cell-dependent humoral responses. APRIL levels are elevated in the serum during coronary artery disease, and it is also elevated in many cancers primarily due to expression by tumor-infiltratin neutrophils. |
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APRIL/TNFSF13 Protein, Human, Recombinant (Flag & His) CD-256 TRDL 1 TRDL-1 TALL2 TALL 2 TNF- and APOL-related leukocyte expressed ligand 2 CD 256 Tumor necrosis factor ligand superfamily member 13 A proliferation-inducing ligand TNF-related death ligand 1 TNFSF13 TNFSF-13 CD256 TRDL1 TNFSF 13 TALL-2 APRIL recombinant recombinant-proteins proteins protein