Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 3,100 | 5日内发货 | ||
500 μg | ¥ 12,400 | 5日内发货 | ||
1 mg | ¥ 20,700 | 5日内发货 |
生物活性 | Immobilized Human Alkaline Phosphatase (Germ type) , His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA. |
产品描述 | Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease. |
种属 | Human |
表达系统 | HEK293 |
标签 | C-His |
蛋白编号 | P10696 |
别名 | ALPG, ALPPL, Alkaline phosphatase, placental-like, Alkaline Phosphatase, ALPPL2, PLAP-like, ALP-1, GCAP |
蛋白构建 | Recombinant Human Alkaline Phosphatase (Germ type) /ALPG Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ile20-Asp503.[Accession |P10696] |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | The protein has a predicted MW of 53.67 kDa. Due to glycosylation, the protein migrates to 58-68 kDa based on Tris-Bis PAGE result. |
内毒素 | Less than 1EU per μg by the LAL method. |
缓冲液 | Lyophilized from 0.22μm filtered solution in 25mM Tris, 100mM NaCl, 1mM MgCl2 (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
复溶方法 | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
存储 |
-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
运输方式 |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
研究背景 | Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease. |
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Alkaline Phosphatase (Germ type) /ALPG Protein, Human, Recombinant (His) ALPG ALPPL Alkaline phosphatase, placental-like ALP 1 Alkaline Phosphatase ALPPL2 ALPPL 2 PLAP-like ALP-1 ALP1 GCAP ALPPL-2 recombinant recombinant-proteins proteins protein