Powder: -20°C for 3 years | In solvent: -80°C for 1 year
NLRP3/AIM2-IN-3 是一种独特的分子,能以物种特异性的方式抑制 NLRP3 和 AIM2 炎性体的激活。它对细胞裂解的 IC50 值为 0.077 ± 0.008 μ M。NLRP3/AIM2-IN-3是一种 NLRP3 和 AIM2 炎症体依赖性细胞裂解的强效抑制剂,对细胞裂解的 IC50 值为 0.077 ± 0.008 μ M。NLRP3/AIM2-IN-3 可抑制 LPS/nigericin 刺激的 THP-1 巨噬细胞的细胞裂解,其 IC50 值为 0.077 ± 0.008 μM。NLRP3/AIM2-IN-3 干扰了 NLRP3 或 AIM2 与桥接蛋白 ASC 的相互作用,抑制了 ASC 的寡聚。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 297 | 现货 | ||
5 mg | ¥ 690 | 现货 | ||
10 mg | ¥ 1,120 | 现货 | ||
25 mg | ¥ 2,320 | 现货 | ||
50 mg | ¥ 3,730 | 现货 | ||
100 mg | ¥ 5,930 | 现货 | ||
500 mg | ¥ 11,900 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 776 | 现货 |
产品描述 | NLRP3/AIM2-IN-3 is a unique molecule that inhibits NLRP3 and AIM2 inflammasome activation in a species-specific manner. It has an IC50 value for cell lysis of 0.077 ± 0.008 μ M. NLRP3/AIM2-IN-3 is a potent inhibitor of NLRP3 and AIM2 inflammasome-dependent cell lysis with an IC50 value for cell lysis of 0.077 ± 0.008 μ M. NLRP3/AIM2-IN-3 inhibits LPS/nigericin NLRP3/AIM2-IN-3 inhibits LPS/nigericin-stimulated cell lysis in THP-1 macrophages with an IC50 value of 0.077 ± 0.008 μM. NLRP3/AIM2-IN-3 interferes with the interaction of NLRP3 or AIM2 with the bridging protein ASC, inhibiting oligomerization of ASC. |
体外活性 |
NLRP3/AIM2-IN-3 (0-100 μM) exhibits significant inhibitory activity against NLRP3 and AIM2, but not NLRC4-dependent activation of caspase-1 and release of IL-1b in human THP-1 macrophages Significant inhibitory effect.[1] NLRP3/AIM2-IN-3 inhibits IL-1b secretion from human THP-1 macrophages with IC50 of 0.098 μM, nearly 150-fold and 500-fold more potent than NLRP3/AIM2-IN-3 for J774A.1 (14.62 μM) and Bone marrow-derived macrophages (BMDM) (48.98 μM).[1] |
分子量 | 266.29 |
分子式 | C16H14N2O2 |
CAS No. | 1787787-60-3 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 225.0 mg/mL (844.9 mM), Sonication is recommended.
Methanol: 45.0 mg/mL (169.0 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO / Methanol | 1 mM | 3.7553 mL | 18.7765 mL | 37.553 mL | 93.8826 mL |
5 mM | 0.7511 mL | 3.7553 mL | 7.5106 mL | 18.7765 mL | |
10 mM | 0.3755 mL | 1.8777 mL | 3.7553 mL | 9.3883 mL | |
20 mM | 0.1878 mL | 0.9388 mL | 1.8777 mL | 4.6941 mL | |
50 mM | 0.0751 mL | 0.3755 mL | 0.7511 mL | 1.8777 mL | |
100 mM | 0.0376 mL | 0.1878 mL | 0.3755 mL | 0.9388 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
NLRP3/AIM2-IN-3 1787787-60-3 Immunology/Inflammation NF-Κb NOD AIM2 Inhibitor inhibitor inhibit