store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
L-NAME hydrochloride (L-NAME HCl) 是NOS 抑制剂(IC50:70 μM)。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
50 mg | ¥ 298 | 现货 | ||
100 mg | ¥ 418 | 现货 | ||
500 mg | ¥ 756 | 现货 |
产品描述 | L-NAME hydrochloride (L-NAME HCl), a cell-permeable NO synthase inhibitor, displays Ki values of 4.4 μM, 15 nM, and 39 nM, for iNOS (mouse) , nNOS (bovine) , and eNOS (human), respectively. |
靶点活性 | nNOS:15 nM(Ki), eNOS:39 nM(Ki) |
体内活性 | L-NAME (0.03-300 mg kg-1, i.v.) induces a dose-dependent increase in mean systemic arterial blood pressure accompanied by bradycardia. L-NAME (100 mg kg-1, i.v.) inhibits significantly the hypotensive responses to ACh and bradykinin. The increase in blood pressure and bradycardia produced by L-NAME is reversed by L-arginine (30-100 mg kg-1, i.v.) in a dose-dependent manner. [2] |
激酶实验 | Enzyme Assay: The oxidation of L-arginine is monitored by the conversion of [3H]- or [14C]-arginine to L-citrulline which separates L-citrulline from L-arginine by Dowex 50x8-200 (Na) chromatography. Typical reaction mixtures (100 pL) contains 50 mM HEPES, pH 7.0, 8 pM tetrahydrobiopterin, 1 mM CaC12, 0.01 mg/mL calmodulin, 0.5 mM EDTA, 0.450 pM [14C]-arginine (30000 cpm), and 100-200 pM NADPH. The cNOS-catalyzed oxidation of NADPH to NADP+ is monitored by the reduction of absorbance at 340 nm with a Kontron 860 spectrophotometer in a volume of 300 pL. All reactions are at 30 ℃ unless otherwise indicated. |
细胞实验 | rMC-1 cells are incubated in 5 or 25 mM glucose, with or without l-NAME (1 mM). Media is changed every other day for up to 5 days. BREC cells are incubated in 5 or 25 mM glucose as well as inhibitor as described above for 5 days. Cell death is determined by light microscopy using a hemocytometer and a 0.4% trypan blue dye exclusion method. The number of cells that do not exclude the dye is expressed per 1,000 total cells. A minimum of 800 cells is counted per assay (8 dishes, >100 cells counted per dish), and the assay is replicated three times on different days. (Only for Reference) |
别名 | L-NAME HCl, N'-硝基-L-精氨酸甲酯盐酸盐, N(G)-Nitro-L-arginine methyl ester, NG-Nitroarginine methyl ester hydrochloride |
分子量 | 269.69 |
分子式 | C7H15N5O4·HCl |
CAS No. | 51298-62-5 |
store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 50 mg/mL (185.4 mM)
H2O: 27 mg/mL (100 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO / H2O | 1 mM | 3.708 mL | 18.5398 mL | 37.0796 mL | 92.699 mL |
5 mM | 0.7416 mL | 3.708 mL | 7.4159 mL | 18.5398 mL | |
10 mM | 0.3708 mL | 1.854 mL | 3.708 mL | 9.2699 mL | |
20 mM | 0.1854 mL | 0.927 mL | 1.854 mL | 4.635 mL | |
50 mM | 0.0742 mL | 0.3708 mL | 0.7416 mL | 1.854 mL | |
100 mM | 0.0371 mL | 0.1854 mL | 0.3708 mL | 0.927 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
L-NAME hydrochloride 51298-62-5 Immunology/Inflammation NO Synthase NOS L-NAME HCl inhibit NG-Nitroarginine methyl ester Hydrochloride N'-硝基-L-精氨酸甲酯盐酸盐 Inhibitor L-NAME Nitric oxide synthases L-NAME Hydrochloride LNAME hydrochloride L NAME hydrochloride NG-Nitroarginine methyl ester N(G)-Nitro-L-arginine methyl ester NG-Nitroarginine methyl ester hydrochloride inhibitor