Ubiquitin carboxyl-terminal hydrolase 7, also known as Ubiquitin thioesterase 7, Herpesvirus-associated ubiquitin-specific protease, Ubiquitin-specific-processing protease 7, USP7 and HAUSP, is a widely expressed protein that belongs to the peptidase C19 family. USP7 is a member of the family of deubiquitinating enzymes. It is involved in the regulation of stress response pathways, epigenetic silencing and the progress of infections by DNA viruses. USP7 is a protein with a cysteine peptidase core, N- and C-terminal domains required for protein-protein interactions. USP7 contributes to epigenetic silencing of homeotic genes by Polycomb (Pc). USP7 cleaves ubiquitin fusion protein substrates. It deubiquitinates TP53/p53 and MDM2 and strongly stabilizes TP53 even in the presence of excess MDM2. USP7 also induces TP53-dependent cell growth repression and apoptosis. USP7 has key roles in the p53 pathway whereby it stabilizes both p53 and MDM2. Herpes simplex virus type 1 (HSV-1) regulatory protein ICP stimulates lytic infection and the reactivation of quiescent viral genomes. ICP interacts very strongly with USP7. USP7-mediated stabilization of ICP is dominant over ICP-induced degradation of USP7 during productive HSV-1 infection. The biological significance of the ICP-USP7 interaction may be most pronounced in natural infection situations, in which limited amounts of ICP are expressed.
生物活性 | Testing in progress |
产品描述 | Ubiquitin carboxyl-terminal hydrolase 7, also known as Ubiquitin thioesterase 7, Herpesvirus-associated ubiquitin-specific protease, Ubiquitin-specific-processing protease 7, USP7 and HAUSP, is a widely expressed protein that belongs to the peptidase C19 family. USP7 is a member of the family of deubiquitinating enzymes. It is involved in the regulation of stress response pathways, epigenetic silencing and the progress of infections by DNA viruses. USP7 is a protein with a cysteine peptidase core, N- and C-terminal domains required for protein-protein interactions. USP7 contributes to epigenetic silencing of homeotic genes by Polycomb (Pc). USP7 cleaves ubiquitin fusion protein substrates. It deubiquitinates TP53/p53 and MDM2 and strongly stabilizes TP53 even in the presence of excess MDM2. USP7 also induces TP53-dependent cell growth repression and apoptosis. USP7 has key roles in the p53 pathway whereby it stabilizes both p53 and MDM2. Herpes simplex virus type 1 (HSV-1) regulatory protein ICP stimulates lytic infection and the reactivation of quiescent viral genomes. ICP interacts very strongly with USP7. USP7-mediated stabilization of ICP is dominant over ICP-induced degradation of USP7 during productive HSV-1 infection. The biological significance of the ICP-USP7 interaction may be most pronounced in natural infection situations, in which limited amounts of ICP are expressed. |
种属 | Human |
表达系统 | Baculovirus-Insect Cells |
标签 | Tag Free |
蛋白编号 | Q93009-1 |
别名 | TEF1, HAUSP, ubiquitin specific peptidase 7 (herpes virus-associated) |
蛋白构建 | A DNA sequence encoding the amino acid sequence (Lys 208-Glu 560) of human USP7 (NP_003461.2) expressed and purified, with two additional aa (Gly & Pro) at the N-terminus. |
蛋白纯度 | > 80 % as determined by SDS-PAGE |
分子量 | Approxiamtely 41 kDa |
内毒素 | < 1.0 EU per μg of the protein as determined by the LAL method |
缓冲液 | Lyophilized from sterile 50mM Tris, 100mM NaCl, 0. 5mM PMSF, pH 8.0. Pleasecon tact usfor any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
复溶方法 | A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information. |
存储 |
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
运输方式 |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
研究背景 | Ubiquitin carboxyl-terminal hydrolase 7, also known as Ubiquitin thioesterase 7, Herpesvirus-associated ubiquitin-specific protease, Ubiquitin-specific-processing protease 7, USP7 and HAUSP, is a widely expressed protein that belongs to the peptidase C19 family. USP7 is a member of the family of deubiquitinating enzymes. It is involved in the regulation of stress response pathways, epigenetic silencing and the progress of infections by DNA viruses. USP7 is a protein with a cysteine peptidase core, N- and C-terminal domains required for protein-protein interactions. USP7 contributes to epigenetic silencing of homeotic genes by Polycomb (Pc). USP7 cleaves ubiquitin fusion protein substrates. It deubiquitinates TP53/p53 and MDM2 and strongly stabilizes TP53 even in the presence of excess MDM2. USP7 also induces TP53-dependent cell growth repression and apoptosis. USP7 has key roles in the p53 pathway whereby it stabilizes both p53 and MDM2. Herpes simplex virus type 1 (HSV-1) regulatory protein ICP stimulates lytic infection and the reactivation of quiescent viral genomes. ICP interacts very strongly with USP7. USP7-mediated stabilization of ICP is dominant over ICP-induced degradation of USP7 during productive HSV-1 infection. The biological significance of the ICP-USP7 interaction may be most pronounced in natural infection situations, in which limited amounts of ICP are expressed. |
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USP7 Protein, Human, Recombinant (aa 208-560) TEF1 herpes virus-associated HAUSP ubiquitin specific peptidase 7 ubiquitin specific peptidase 7 (herpes virus-associated) TEF 1 TEF-1 recombinant recombinant-proteins proteins protein