5T4/TPBG Protein, Human, Recombinant (mFc)

5T4/TPBG Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with C-mFc tag. The predicted molecular weight is 80-110 KDa and the accession number is Q13641.

规格	价格/CNY		数量
10 μg	￥ 830	5日内发货
50 μg	￥ 2,510	5日内发货
500 μg	￥ 12,600	5日内发货
1 mg	￥ 18,900	5日内发货

规格

价格/CNY

货期

数量

10 μg

￥ 830

5日内发货

50 μg

￥ 2,510

5日内发货

500 μg

￥ 12,600

5日内发货

1 mg

￥ 18,900

5日内发货

生物活性	1. Loaded Human TPBG Antibody (5G9) (Human IgG1) on Protein-A Biosensor, can bind Human TPBG (C-mFc) with an affinity constant of 8.75 nM as determined in BLI assay. (Regularly tested) 2. Immobilized Human TPBG (C-mFc) at 2μg/ml (100 μl/well) can bind Human TPBG Antibody (5G9) (Human IgG1) . The ED50 of Human TPBG Antibody (5G9) (Human IgG1) is 1.57 ng/ml. (Regularly tested)
产品描述	5T4/TPBG Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with C-mFc tag. The predicted molecular weight is 80-110 KDa and the accession number is Q13641.

种属	Human
表达系统	Human Cells
标签	C-mFc
蛋白编号	Q13641
别名	TPBG, 5T4 Protein, M6P1 Protein, 5T4AG Protein, WAIF1 Protein
氨基酸序列	Ser32-Ser355
蛋白构建	Recombinant Human Trophoblast glycoprotein is produced by our Mammalian expression system and the target gene encoding Ser32-Ser355 is expressed with a mouse IgG1 Fc tag at the C-terminus.
蛋白纯度	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
分子量	80-110 KDa, reducing conditions

内毒素	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
缓冲液	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
复溶方法	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
存储	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
运输方式	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
研究背景	Trophoblast glycoprotein, also known as TPBG and 5T4, it belongs to the LRR family of proteins. The C-terminus of LRR N-terminal cap (LRRNT) and LRR 1 are essential for the inhibition of the Wnt signaling pathway. TPBG may function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization. It expressed by all types of trophoblasts as early as 9 weeks of development. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma.

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5T4/TPBG Protein, Human, Recombinant (mFc)

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