TNFSF15 Protein, Mouse, Recombinant (hFc) is expressed in HEK293 mammalian cells with N-hFc tag. The predicted molecular weight is 48.8 kDa and the accession number is Q5UBV8.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 3,100 | 5日内发货 | ||
500 μg | ¥ 12,400 | 5日内发货 | ||
1 mg | ¥ 20,700 | 5日内发货 |
生物活性 | 1. Immobilized Mouse DR3, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Mouse TNFSF15, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA. 2. Mouse TNFSF15, hFc Tag captured on CM5 Chip via Protein A can bind Mouse DR3, His Tag with an affinity constant of 4.56 nM as determined in SPR assay. |
产品描述 | TNFSF15 Protein, Mouse, Recombinant (hFc) is expressed in HEK293 mammalian cells with N-hFc tag. The predicted molecular weight is 48.8 kDa and the accession number is Q5UBV8. |
种属 | Mouse |
表达系统 | HEK293 |
标签 | N-hFc |
蛋白编号 | Q5UBV8 |
别名 | VEGI192A, TL1, VEGI-251, TL1A, TNFSF15, VEGI |
蛋白构建 | Recombinant Mouse TNFSF15 Protein is expressed from HEK293 with hFc tag at the N-Terminus. It contains Ala61-Leu252.[Accession |Q5UBV8] |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | The protein has a predicted MW of 48.8 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result. |
内毒素 | Less than 1EU per μg by the LAL method. |
缓冲液 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
复溶方法 | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
存储 |
-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
运输方式 |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
研究背景 | TL1A and its functional receptor DR3 are members of the TNF/TNFR superfamilies of proteins.TL1A and DR3 are abundantly localized at inflamed intestinal areas of patients with IBD and mice with experimental ileitis or colitis and actively participate in the immunological pathways that underlie mucosal homeostasis and intestinal inflammation.Recently, an important role was demonstrated for TL1A/DR3 as potential mediators of intestinal fibrosis that is associated with the presence of gut inflammation. |
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TL1A/TNFSF15 Protein, Mouse, Recombinant (hFc) VEGI 251 TNFSF 15 VEGI192A TL1 VEGI-251 TL1A TL-1 TNFSF15 TNFSF-15 VEGI251 VEGI TL 1 recombinant recombinant-proteins proteins protein