Src Protein, Mouse, Recombinant (His & GST)

Proto-oncogene tyrosine-protein kinase SRC is a hydrophobic protein belonging to the SRC family kinase including nine members that is a family of non-receptor tyrosine kinases. SRC protein may exist in different forms: C-SRC and V-SRC. C-SRC is only activated under certain circumstances where it is required such as growth factor signaling, while V-SRC is constitutively active as opposed to normal SRC (C-SRC). Thus, V-SRC is an instructive example of an oncogene protein kinase whereas C-SRC is a proto-oncogene protein kinase. Inhibition of SRC with NR2A tyrosine phosphorylation mediated by PSD-95 may contribute to the lithium-induced downregulation of NMDA receptor function and provide neuroprotection against excitotoxicity.

规格	价格/CNY		货期	数量
50 μg	￥ 3,170	5日内发货

规格

价格/CNY

货期

数量

50 μg

￥ 3,170

5日内发货

生物活性

1. The specific activity was determined to be ＞80 nmol/min/mg using poly [Glu, Tyr] 4:1 as substrate. 2. Measured by its binding ability in a functional ELISA. Immobilized recombinant Mouse SRC at 2 μg/ml (100 μl/well) can bind biotinylated human PTPRA (aa 174-793) with a linear range of 0.032-0.8 μg/ml.

产品描述

种属	Mouse
表达系统	Baculovirus-Insect Cells
标签	His,GST
蛋白编号	NP_001020566.111
别名	SRC proto-oncogene, non-receptor tyrosine kinase, AW259666, pp60c-src
蛋白构建	A DNA sequence encoding the mouse SRC (NP_001020566.111) (Met 1-Leu 535) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
蛋白纯度	> 90 % as determined by SDS-PAGE
分子量	87.7 kDa (predicted)

内毒素	< 1.0 EU per μg of the protein as determined by the LAL method
缓冲液	Supplied as sterile 20mM Tris, 500mM NaCl, 10% gly, pH 8.0. Pleasecon tact usfor any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.
复溶方法	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
存储	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
运输方式	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.
研究背景	Proto-oncogene tyrosine-protein kinase SRC is a hydrophobic protein belonging to the SRC family kinase including nine members that is a family of non-receptor tyrosine kinases. SRC protein may exist in different forms: C-SRC and V-SRC. C-SRC is only activated under certain circumstances where it is required such as growth factor signaling, while V-SRC is constitutively active as opposed to normal SRC (C-SRC). Thus, V-SRC is an instructive example of an oncogene protein kinase whereas C-SRC is a proto-oncogene protein kinase. Inhibition of SRC with NR2A tyrosine phosphorylation mediated by PSD-95 may contribute to the lithium-induced downregulation of NMDA receptor function and provide neuroprotection against excitotoxicity.