SARS-COV-2 Spike S1 Protein (D614G, His & Avi)

The SARS-CoV-2 spike (S) protein is the target of vaccine design efforts to end the COVID-19 pandemic. Despite a low mutation rate, isolates with the D614G substitution in the S protein appeared early during the pandemic, and are now the dominant form worldwide. Here, we analyze the D614G mutation in the context of a soluble S ectodomain construct.

规格	价格/CNY		数量
100 μg	￥ 3,250	5日内发货
500 μg	￥ 13,000	5日内发货
1 mg	￥ 21,700	5日内发货

规格

价格/CNY

货期

数量

100 μg

￥ 3,250

5日内发货

500 μg

￥ 13,000

5日内发货

1 mg

￥ 21,700

5日内发货

生物活性	Immobilized SARS-CoV-2 S1 (D614G) , His Tag at 2μg/ml (100μl/Well) on plate.Dose response curve for Human ACE2, hFc Tag with the EC50 of 23.7ng/ml determined by ELISA.
产品描述	The SARS-CoV-2 spike (S) protein is the target of vaccine design efforts to end the COVID-19 pandemic. Despite a low mutation rate, isolates with the D614G substitution in the S protein appeared early during the pandemic, and are now the dominant form worldwide. Here, we analyze the D614G mutation in the context of a soluble S ectodomain construct.

种属	SARS-CoV-2
表达系统	HEK293
标签	C-His-Avi
蛋白编号	A0A6G7K2L4
别名	Spike,S1 protein, Spike protein S1, S glycoprotein Subunit1, S1 protein
蛋白构建	Recombinant SARS-COV-2 Spike S1 (D614G) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gln14-Arg683(D614G).[Accession \|QHD43416.1]
蛋白纯度	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result.

内毒素	Less than 1EU per μg by the LAL method.
缓冲液	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶方法	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
存储	-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
运输方式	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
研究背景	The SARS-CoV-2 spike (S) protein is the target of vaccine design efforts to end the COVID-19 pandemic. Despite a low mutation rate, isolates with the D614G substitution in the S protein appeared early during the pandemic, and are now the dominant form worldwide. Here, we analyze the D614G mutation in the context of a soluble S ectodomain construct.