SOST Protein, Rat, Recombinant (His)

Sclerostin, the protein product of the SOST gene, is a potent inhibitor of bone formation. Sclerostin protein is widely expressed at low levels with highest levels in bone, cartilage, kidney, liver, bone marrow and primary osteeoblasts differentiated for 21 days, and was originally identified as an important regulator of bone remodeling, homeostasis, and links bone resorption and bone apposition. Recent studies have revealed that Sclerostin protein inhibits the bone growth probably by binding to the extracellular domain of the Wnt coreceptors LRP5 and LRP6 and disrupting Wnt-induced Frizzled-LRP complex formation.

规格	价格/CNY		数量
20 μg	￥ 1,950	现货
100 μg	￥ 6,740	5日内发货
200 μg	￥ 11,490	5日内发货
500 μg	￥ 23,220	5日内发货

规格

价格/CNY

货期

数量

20 μg

￥ 1,950

现货

100 μg

￥ 6,740

5日内发货

200 μg

￥ 11,490

5日内发货

500 μg

￥ 23,220

5日内发货

生物活性

Measured by its ability to inhibit Wnt3a induced alkaline phosphatase production by C3H10 T1/2 cells. The ED50 for this effect is typically 0.1-0.5 μg/mL.

产品描述

种属	Rat
表达系统	HEK293
标签	His
蛋白编号	A6HJF6
别名	sclerostin
蛋白构建	A DNA sequence encoding the rat SOST (NP_085073.1) (Met 1-Tyr 213) was expressed, fused with a polyhistidine tag at the C-terminus.
蛋白纯度	> 97 % as determined by SDS-PAGE
分子量	23 kDa (predicted)

内毒素	< 1.0 EU per μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
复溶方法	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
存储	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
运输方式	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
研究背景	Sclerostin, the protein product of the SOST gene, is a potent inhibitor of bone formation. Sclerostin protein is widely expressed at low levels with highest levels in bone, cartilage, kidney, liver, bone marrow and primary osteeoblasts differentiated for 21 days, and was originally identified as an important regulator of bone remodeling, homeostasis, and links bone resorption and bone apposition. Recent studies have revealed that Sclerostin protein inhibits the bone growth probably by binding to the extracellular domain of the Wnt coreceptors LRP5 and LRP6 and disrupting Wnt-induced Frizzled-LRP complex formation.