SMAD3 Protein, Human, Recombinant (His & Flag)

Mothers against decapentaplegic homolog 3(SMAD3) is a cytoplasm protein which belongs to the dwarfin/SMAD family. Smad proteins undergo rapid nuclear translocation upon stimulation by transforming growth factor and in so doing transduce the signal into the nucleus. Receptor-regulated SMAD is an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. SMAD3 binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. It also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. SMAD3 has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive.

规格	价格/CNY		数量
10 μg	￥ 700	5日内发货
50 μg	￥ 1,970	5日内发货
500 μg	￥ 8,860	5日内发货
1 mg	￥ 12,600	5日内发货

规格

价格/CNY

货期

数量

10 μg

￥ 700

5日内发货

50 μg

￥ 1,970

5日内发货

500 μg

￥ 8,860

5日内发货

1 mg

￥ 12,600

5日内发货

产品描述

种属	Human
表达系统	E. coli
标签	N-6His-Flag
蛋白编号	P84022
别名	SMAD 3, Mothers against DPP homolog 3, hMAD-3, MADH3, JV15-2, SMAD family member 3, SMAD3, MAD homolog 3, Mothers against decapentaplegic homolog 3, hSMAD3, Mad3
氨基酸序列	Ser2-Ser425
蛋白构建	Recombinant Human Mothers Against Decapentaplegic Homolog 3 is produced by our E.coli expression system and the target gene encoding Ser2-Ser425 is expressed with a 6His, Flag tag at the N-terminus.
蛋白纯度	Greater than 85% as determined by reducing SDS-PAGE. (QC verified)
分子量	50-60 KDa, reducing conditions

内毒素	Less than 1 EU/µg as determined by LAL test.
缓冲液	Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 500mM NaCl, 10% Glycerol, 2mM EDTA, pH 8.0.
存储	Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
运输方式	The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.
研究背景	Mothers against decapentaplegic homolog 3(SMAD3) is a cytoplasm protein which belongs to the dwarfin/SMAD family. Smad proteins undergo rapid nuclear translocation upon stimulation by transforming growth factor and in so doing transduce the signal into the nucleus. Receptor-regulated SMAD is an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. SMAD3 binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. It also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. SMAD3 has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive.

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SMAD3 Protein, Human, Recombinant (His & Flag)

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