Siglec-2/CD22 Protein, Human, Recombinant (hFc)

Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains. Human Siglec-2, also known as B-cell antigen CD22 or B-lymphocyte cell adhesion molecule (BL-CAM), is a B-cell restricted glycoprotein that is expressed in the cytoplasm of progenitor B and pre-B cells and on the surface of mature B cells. Two distinct human Siglec-2/CD22 cDNAs that arise from differential RNA processing of the same gene have been isolated. Siglec-2/CD22 is an adhesion molecule that preferentially binds alpha 2,6- linked sialic acid on the same (cis) or adjacent (trans) cells. Interaction of CD22 with trans ligands on opposing cells was found to be favored over the binding of ligands in cis.

规格	价格/CNY		数量
10 μg	￥ 820	5日内发货
50 μg	￥ 2,480	5日内发货
500 μg	￥ 12,100	5日内发货
1 mg	￥ 17,400	5日内发货

规格

价格/CNY

货期

数量

10 μg

￥ 820

5日内发货

50 μg

￥ 2,480

5日内发货

500 μg

￥ 12,100

5日内发货

1 mg

￥ 17,400

5日内发货

生物活性

Immobilized Anti-Human Siglec-2 mAb at 2μg/ml (100 μl/well)can bind Human Siglec-2-Fc. The ED50 of Human Siglec-2-Fc is 24.05 ng/ml. (Regularly tested)

产品描述

种属	Human
表达系统	Human Cells
标签	C-Fc
蛋白编号	P20273
别名	BL-CAM, SIGLEC2FLJ22814, CD22 antigenMGC130020, sialic acid binding Ig-like lectin 2, B-lymphocyte cell adhesion molecule, T-cell surface antigen Leu-14, Siglec-2, CD22, B-cell receptor CD22, CD22 molecule
氨基酸序列	Asp20-Arg687
蛋白构建	Recombinant Human B-cell Receptor CD22 is produced by our Mammalian expression system and the target gene encoding Asp20-Arg687 is expressed with a human IgG1 Fc tag at the C-terminus.
蛋白纯度	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
分子量	120-150 KDa, reducing conditions

内毒素	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
缓冲液	Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 1mM EDTA, 150mM NaCl, pH 8.0 .
复溶方法	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
存储	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
运输方式	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
研究背景	Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains. Human Siglec-2, also known as B-cell antigen CD22 or B-lymphocyte cell adhesion molecule (BL-CAM), is a B-cell restricted glycoprotein that is expressed in the cytoplasm of progenitor B and pre-B cells and on the surface of mature B cells. Two distinct human Siglec-2/CD22 cDNAs that arise from differential RNA processing of the same gene have been isolated. Siglec-2/CD22 is an adhesion molecule that preferentially binds alpha 2,6- linked sialic acid on the same (cis) or adjacent (trans) cells. Interaction of CD22 with trans ligands on opposing cells was found to be favored over the binding of ligands in cis.

计算器

复溶计算器

重组蛋白稀释计算器

比活力计算器

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

bottom

Siglec-2/CD22 Protein, Human, Recombinant (hFc)

计算器

技术支持

Keywords