Siglec-10 Protein, Mouse, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-Fc tag. The predicted molecular weight is 110-135 KDa and the accession number is Q80ZE3.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
10 μg | ¥ 650 | 5日内发货 | ||
50 μg | ¥ 1,970 | 5日内发货 | ||
500 μg | ¥ 14,200 | 5日内发货 | ||
1 mg | ¥ 21,300 | 5日内发货 |
产品描述 | Siglec-10 Protein, Mouse, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-Fc tag. The predicted molecular weight is 110-135 KDa and the accession number is Q80ZE3. |
种属 | Mouse |
表达系统 | HEK293 Cells |
标签 | C-hFc |
蛋白编号 | Q80ZE3 |
别名 | MGC126774, SLG2, SiglecG, Siglec-10, sialic acid-binding Ig-like lectin 10, Siglec10, PRO940, siglec-like gene 2, Siglec-like protein 2, Siglec-G, SLG2sialic acid binding Ig-like lectin 10 Ig-like lectin 7 |
氨基酸序列 | Met19-Lys543 |
蛋白构建 | Met19-Lys543 |
蛋白纯度 | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
分子量 | 110-135 KDa (reducing condition) |
内毒素 | < 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 |
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
研究背景 | Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system. Siglec-G is the apparent ortholog of human Siglec-10. We describe here a novel member of the siglec protein family that shares a similar structure including five Ig-like domains, a transmembrane domain, and a cytoplasmic tail containing two ITIM-signaling motifs. Siglec-10 was identified through database mining of an asthmatic eosinophil EST library. Siglec-10 binds sialated proteins and lipids in alpha 2,3 or alpha 2,6 linkage and shows a preference for GT1b gangliosides. This binding can be modulated by cis interactions of Siglec-10 with sialated molecules expressed on the same cell. When tyrosine phosphorylated, the cytoplasmic ITIMs interact with phosphatases SHP-1 and SHP-2 to propagate inhibitory signals. The Siglec-10-VAP-1 interaction seems to mediate lymphocyte adhesion to endothelium and has the potential to modify the inflammatory microenvironment via the enzymatic end products. |
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Siglec-10 Protein, Mouse, Recombinant (hFc) MGC126774 SLG2 SiglecG SLG-2 PRO 940 Siglec-10 sialic acid-binding Ig-like lectin 10 Siglec10 PRO940 siglec-like gene 2 MGC-126774 Siglec-like protein 2 Siglec-G SLG 2 PRO-940 MGC 126774 SLG2sialic acid binding Ig-like lectin 10 Ig-like lectin 7 recombinant recombinant-proteins proteins protein