The Bacillus subtilis enzyme Sfp, required for production of the lipoheptapeptide antibiotic surfactin, posttranslationally phosphopantetheinylates a serine residue in each of the seven peptidyl carrier protein domains of the first three subunits (SrfABC) of surfactin synthetase to yield docking sites for amino acid loading and peptide bond formation.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
10 μg | ¥ 930 | 5日内发货 | ||
50 μg | ¥ 2,810 | 5日内发货 | ||
500 μg | ¥ 13,100 | 5日内发货 | ||
1 mg | ¥ 19,700 | 5日内发货 |
产品描述 | The Bacillus subtilis enzyme Sfp, required for production of the lipoheptapeptide antibiotic surfactin, posttranslationally phosphopantetheinylates a serine residue in each of the seven peptidyl carrier protein domains of the first three subunits (SrfABC) of surfactin synthetase to yield docking sites for amino acid loading and peptide bond formation. |
种属 | B.subtilis |
表达系统 | E. coli |
标签 | C-6His |
蛋白编号 | CAA44858.1 |
别名 | Surfactin synthase-activating enzyme, sfp, lpa-8, 4'-phosphopantetheinyl transferase sfp |
氨基酸序列 | Met1-Leu224 |
蛋白构建 | Recombinant B.subtilis 4'-phosphopantetheinyl Transferase Sfp/Sfp is produced by our E.coli expression system and the target gene encoding Met1-Leu224 is expressed with a 6His tag at the C-terminus. |
蛋白纯度 | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
分子量 | 28 KDa, reducing conditions |
内毒素 | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
缓冲液 | Supplied as a 0.2 μm filtered solution of 20mM PB, 100mM NaCl, pH 7.0. |
存储 |
Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
运输方式 |
The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below. |
研究背景 | The Bacillus subtilis enzyme Sfp, required for production of the lipoheptapeptide antibiotic surfactin, posttranslationally phosphopantetheinylates a serine residue in each of the seven peptidyl carrier protein domains of the first three subunits (SrfABC) of surfactin synthetase to yield docking sites for amino acid loading and peptide bond formation. |
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SFP Protein, B.subtilis, Recombinant (His) Surfactin synthase-activating enzyme sfp lpa-8 4'-phosphopantetheinyl transferase sfp recombinant recombinant-proteins proteins protein