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SAE1 Protein, Human, Recombinant (His & GST)

产品编号 TMPY-03062

别名: UBLE1A, SUMO1 activating enzyme subunit 1, AOS1, HSPC140, SUA1

SAE1 Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 66.3 kDa and the accession number is Q9UBE0.

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SAE1 Protein, Human, Recombinant (His & GST)

规格	价格/CNY		货期	数量
100 μg	￥ 3,820		5日内发货

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生物活性	Testing in progress
产品描述	SAE1 Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 66.3 kDa and the accession number is Q9UBE0.

种属	Human
表达系统	Baculovirus Insect Cells
标签	His, GST
蛋白编号	Q9UBE0
别名	UBLE1A, SUMO1 activating enzyme subunit 1, AOS1, HSPC140, SUA1
蛋白构建	The Human SAE1 (Q9UBE0) (Met1-Lys346) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
蛋白纯度	> 95 % as determined by SDS-PAGE
分子量	66.3 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, pH 7.4, 10% gly. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	SAE1 belongs to the ubiquitin-activating E1 family. It is a heterodimer that acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It functions as a UBLI E1 ligase mediating the ATP-dependent activation of UBL1. SAE1 binds with UBLE1A and UBLE1B to form a heterodimer which can bind UBL1. SAE1 also regulates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins.

参考文献

1. Gong L, et al. (1999) Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex. FEBS Lett. 448(1):185-9. 2. Lois LM, et al. (2005) Structures of the SUMO E1 provide mechanistic insights into SUMO activation and E2 recruitment to EEMBO J. 24(3):439-51. 3. Tatham MH, et al. (2001) Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein substrates by SAE1/SAE2 and Ubc9. J Biol Chem. 276(38):35368-74.

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Keywords

SAE1 Protein, Human, Recombinant (His & GST) HSPC-140 HSPC 140 SUA-1 UBLE1A SUMO1 activating enzyme subunit 1 AOS1 AOS 1 HSPC140 SUA1 AOS-1 SUA 1 recombinant recombinant-proteins proteins protein

我们的所有产品和服务仅用于科学研究，不能被用于人体，我们也不向个人提供产品和服务。

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