PU.1/SPI1 Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 32.04 kDa and the accession number is P17947.
生物活性 | Testing in progress |
产品描述 | PU.1/SPI1 Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 32.04 kDa and the accession number is P17947. |
种属 | Human |
表达系统 | E. coli |
标签 | His |
蛋白编号 | P17947 |
别名 | SFPI1, SPI-A, spleen focus forming virus (SFFV) proviral integration oncogene, PU.1, SPI-1, OF |
蛋白构建 | A DNA sequence encoding the Human SPI1 (P17947) (Met1-His270) was expressed, with a polyhistidine tag at the N-terminus. |
蛋白纯度 | ≥ 90 % as determined by SDS-PAGE. |
分子量 | 32.04 kDa (predicted) |
内毒素 | < 1.0 EU per μg protein as determined by the LAL method. |
缓冲液 | Lyophilized from sterile PBS, 0. 05% SKL. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
复溶方法 | A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information. |
存储 |
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
运输方式 |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
研究背景 | SPI1 (also known as PU.1) is a dominant but transient regulator in early T-cell precursors and a potent transcriptional controller of developmentally important pro-T-cell genes. SPI1 is an essential transcription factor (TF) for the hematopoietic lineage, in which its expression is tightly controlled through a -17-kb upstream regulatory region and a promoter region. The E26 transformation-specific (ETS) family transcription factor PU.1/Spi1 acts as a master regulator of myeloid and lymphoid development. PU.1-deficient mice show a complete loss of microglia, indicating that PU.1 plays a pivotal role in micro-gliogenesis. The Spi1/PU.1 transcription factor is a key regulator of many steps of hematopoiesis, and limits self-renewal of hematopoietic stem cells. The deregulation of its expression or activity contributes to leukemia, in which Spi1 can be either an oncogene or a tumor suppressor. |
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PU.1/SPI1 Protein, Human, Recombinant (His) SFPI1 SPI-A spleen focus forming virus (SFFV) proviral integration oncogene SFPI 1 SFPI-1 SPI1 PU.1 SPI-1 SPI 1 OF recombinant recombinant-proteins proteins protein