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PLBL2/PLBD2 Protein, Chinese hamster, Recombinant (His)

产品编号 TMPY-06116

别名: phospholipase B domain containing 2

PLBL2/PLBD2 Protein, Chinese hamster, Recombinant (His) is expressed in CHO mammalian cells with His tag. The predicted molecular weight is 63.3 kDa and the accession number is G3I6T1-1.

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PLBL2/PLBD2 Protein, Chinese hamster, Recombinant (His)

规格	价格/CNY		货期	数量
100 μg	￥ 5,100		5日内发货
1 mg	￥ 33,200		5日内发货

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参考文献

生物活性	Testing in progress
产品描述	PLBL2/PLBD2 Protein, Chinese hamster, Recombinant (His) is expressed in CHO mammalian cells with His tag. The predicted molecular weight is 63.3 kDa and the accession number is G3I6T1-1.

种属	Chinese hamster
表达系统	CHO Cells
标签	C-His
蛋白编号	G3I6T1-1
别名	phospholipase B domain containing 2
蛋白构建	The chinese hamster PLBD2 (G3I6T1-1) (Leu38-Asp585) was expressed with a polyhistidine tag at the C-terminus.
蛋白纯度	≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
分子量	63.3 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	PLBD2 localizes to the lysosome, as its absence could plausibly lead to a serious yet unrecognized lysosomal storage disease. PLBD1 and PLBD2 are semi-orphans in the sense of being probable phospholipases of B class but with uncertain physiological substrates and thus functionalities. PLBD1 and PLBD2 constitute a small gene family (sequence homology class) within vertebrates though one that occurs expanded in some early diverging eukaryotes. PLBD2 presents a special difficulty in that a sequence of post-translational steps are apparently necessary for its activation. Without these, potential substrates can hardly be assayed. These steps include removal of the signal peptide, mannosylation appropriate to the lysosome targeting receptor, and self-catalytic proteolytic activation to expose the substrate binding site as this becomes appropriate.

参考文献

1. Morgan CP. et al., 2004), Biochem J. 382 (2): 441-9. 2. Kim W. et al., 2011, Mol Cell. 44 (2): 325-40. 3. Havugimana PC. et al., 2012. Cell. 150 (5): 1068-81.

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Keywords

PLBL2/PLBD2 Protein, Chinese hamster, Recombinant (His) phospholipase B domain containing 2 recombinant recombinant-proteins proteins protein

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