PD-L1 Protein, Human, Recombinant (hFc & Avi), Biotinylated

CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.

规格	价格/CNY		货期	数量
20 μg	￥ 2,260	5日内发货
100 μg	￥ 6,130	5日内发货

规格

价格/CNY

货期

数量

20 μg

￥ 2,260

5日内发货

100 μg

￥ 6,130

5日内发货

生物活性

1. Loaded Mouse PD-1-His on HIS1K Biosensor, can bind Biotinylated Human PD-L1-Fc-Avi with an affinity constant of <10^-3 nM as determined in BLI assay. (Regularly tested) 2. Immobilized Recombinant Human PD-1-His at 5μg/ml (100 μl/well)can bind Human PD-L1-Fc-Avi. The ED50 of Human PD-L1-Fc-Avi is 0.185 ug/ml. (Regularly tested)

产品描述

种属	Human
表达系统	Human Cells
标签	C-Fc-Avi
蛋白编号	Q9NZQ7
别名	B7-H1, PDCD1LG1, PD-L1, PDCD1L1, B7 homolog 1, Programmed death ligand 1, B7H1, PDCD1 ligand 1, CD274, PDL1, Programmed Cell Death 1 Ligand 1
氨基酸序列	Phe19-Thr239
蛋白构建	Biotinylated Recombinant Human Programmed Cell Death 1 Ligand 1 is produced by our Mammalian expression system and the target gene encoding Phe19-Thr239 is expressed with a human IgG1 Fc, Avi tag at the C-terminus.
蛋白纯度	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
分子量	70-95 KDa, reducing conditions

内毒素	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
缓冲液	Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
复溶方法	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
存储	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
运输方式	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
研究背景	CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.

计算器

复溶计算器

重组蛋白稀释计算器

比活力计算器

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

bottom

PD-L1 Protein, Human, Recombinant (hFc & Avi), Biotinylated

计算器

技术支持

Keywords