PCK2 promotes tumor initiation by lowering acetyl-CoA level through reducing the mitochondrial tricarboxylic acid (TCA) cycle. The levels of phosphoenolpyruvate carboxykinase isoform 2 (PCK2) are critical for the metabolic switch and the maintenance of TICs in prostate cancer. PCK2 is a potential therapeutic target for aggressive prostate tumors.
生物活性 | Kinase activity untested |
产品描述 | PCK2 promotes tumor initiation by lowering acetyl-CoA level through reducing the mitochondrial tricarboxylic acid (TCA) cycle. The levels of phosphoenolpyruvate carboxykinase isoform 2 (PCK2) are critical for the metabolic switch and the maintenance of TICs in prostate cancer. PCK2 is a potential therapeutic target for aggressive prostate tumors. |
种属 | Human |
表达系统 | Baculovirus-Insect Cells |
标签 | His,GST |
蛋白编号 | A0A384MTT2 |
别名 | PEPCK-M, PEPCK2, PEPCK, phosphoenolpyruvate carboxykinase 2 (mitochondrial) |
蛋白构建 | A DNA sequence encoding the human PCK2 (NP_004554.3) (Leu33-Met640) was expressed with a C-terminal polyhistidine-tagged GST tag at the N-terminus (his-GST). |
蛋白纯度 | > 90 % as determined by SDS-PAGE. |
分子量 | 94.9 kDa (predicted) |
内毒素 | < 1.0 EU per μg protein as determined by the LAL method. |
缓冲液 | Supplied as sterile 20 mM Tris, 500 mM NaCl, 10 %glycerol, pH 8.0. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA. |
复溶方法 | A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. |
存储 |
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
运输方式 |
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. |
研究背景 | PCK2 promotes tumor initiation by lowering acetyl-CoA level through reducing the mitochondrial tricarboxylic acid (TCA) cycle. The levels of phosphoenolpyruvate carboxykinase isoform 2 (PCK2) are critical for the metabolic switch and the maintenance of TICs in prostate cancer. PCK2 is a potential therapeutic target for aggressive prostate tumors. |
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PCK2 Protein, Human, Recombinant (His & GST) PEPCK-M PEPCK2 phosphoenolpyruvate carboxykinase 2 PEPCK PEPCK 2 mitochondrial phosphoenolpyruvate carboxykinase 2 (mitochondrial) PEPCK-2 recombinant recombinant-proteins proteins protein