Osteoactivin/GPNMB Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 52.2 kDa and the accession number is Q14956-2.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 3,820 | 5日内发货 | ||
1 mg | ¥ 24,900 | 5日内发货 |
生物活性 | Testing in progress |
产品描述 | Osteoactivin/GPNMB Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 52.2 kDa and the accession number is Q14956-2. |
种属 | Human |
表达系统 | HEK293 |
标签 | His |
蛋白编号 | Q14956-2 |
别名 | HGFIN, glycoprotein (transmembrane) nmb, Osteoactivin, NMB |
蛋白构建 | A DNA sequence encoding the human GPNMB isoform 2 (Q14956-2) extracellular domain (Met 1-Pro 474) was fused with a polyhistidine tag at the C-terminus. |
蛋白纯度 | ≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. |
分子量 | 52.2 kDa (predicted) |
内毒素 | < 1.0 EU per μg of the protein as determined by the LAL method |
缓冲液 | Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
复溶方法 | A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information. |
存储 |
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
运输方式 |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
研究背景 | GPNMB belongs to the PMEL / NMB family, also known as Osteoactivin and Hematopoietic growth factor-inducible neurokinin 1 ( HGFIN ), is a transmembrane glycoprotein that is expressed in numerous cells, including osteoclasts, macrophages, dendritic cells, and tumor cells. It is suggested to influence osteoblast maturation, cell adhesion, and migration. GPNMB protein acts as a downstream mediator of BMP-2 effects on osteoblast differentiation and function. GPNMB participates in bone mineralization and functions as a negative regulator of inflammation in macrophages. Osteoactivin is expressed at high levels in normal and inflammatory liver macrophages suggesting a significant role in acute liver injury. The early-phase upregulation of Osteoactivin expression in the tubular epithelium in response to renal injury might play a role in triggering renal interstitial fibrosis via activation of matrix metalloproteinase expression and collagen remodeling in rats. Osteoactivin is a protein that is expressed in aggressive human breast cancers and is capable of promoting breast cancer metastasis to bone. |
bottom
您可能有的问题的答案可以在重组蛋白操作手册中找到
Osteoactivin/GPNMB Protein, Human, Recombinant (His) HGFIN glycoprotein (transmembrane) nmb Osteoactivin NMB recombinant recombinant-proteins proteins protein