NAD(P) transhydrogenase/NNT Protein, Human, Recombinant (His)

NAD(P)+transhydrogenase (NNT) is located in the inner mitochondrial membrane and catalyzes a reversible hydride transfer between NAD(H) and NADP(H) that is coupled to proton translocation between the intermembrane space and mitochondrial matrix. NNT activity has an essential role in maintaining the NADPH supply for antioxidant defense and biosynthetic pathways. Structurally, NNT is composed of three domains; domains I and III are hydrophilic and have binding sites for NAD and NADP, respectively, while domain II is hydrophobic and is a transmembrane pathway through which protons translocate. NNT forms dimers, whose monomers act in an anti-phase way; domain III (NADP(H)- binding) flips, allowing proton translocation across the inner mitochondrial membrane one moment and favoring hydride transfer between NAD(H) and NADP(H) the next. And NNT pathophysiological roles after the discovery of a spontaneous Nnt mutation in C57BL/6J mice. And Nnt silencing reduced the growth of cancer cell lines, suggesting that NNT might be a therapeutic target in some cancers.

规格	价格/CNY		数量
10 μg	￥ 750	5日内发货
50 μg	￥ 2,260	5日内发货
500 μg	￥ 12,800	5日内发货
1 mg	￥ 19,200	5日内发货

规格

价格/CNY

货期

数量

10 μg

￥ 750

5日内发货

50 μg

￥ 2,260

5日内发货

500 μg

￥ 12,800

5日内发货

1 mg

￥ 19,200

5日内发货

产品描述

种属	Human
表达系统	E. coli
标签	N-6His
蛋白编号	Q13423
别名	NAD(P) transhydrogenase, NNT
氨基酸序列	Met880-Lys1086
蛋白构建	Recombinant Human NAD(P) transhydrogenase is produced by our E.coli expression system and the target gene encoding Met880-Lys1086 is expressed with a 6His tag at the N-terminus.
蛋白纯度	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
分子量	30 KDa, reducing conditions

内毒素	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
缓冲液	Supplied as a 0.2 μm filtered solution of PBS, 10mM GSH, 50% Glycerol, pH7.4.
存储	Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
运输方式	The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.
研究背景	NAD(P)+transhydrogenase (NNT) is located in the inner mitochondrial membrane and catalyzes a reversible hydride transfer between NAD(H) and NADP(H) that is coupled to proton translocation between the intermembrane space and mitochondrial matrix. NNT activity has an essential role in maintaining the NADPH supply for antioxidant defense and biosynthetic pathways. Structurally, NNT is composed of three domains; domains I and III are hydrophilic and have binding sites for NAD and NADP, respectively, while domain II is hydrophobic and is a transmembrane pathway through which protons translocate. NNT forms dimers, whose monomers act in an anti-phase way; domain III (NADP(H)- binding) flips, allowing proton translocation across the inner mitochondrial membrane one moment and favoring hydride transfer between NAD(H) and NADP(H) the next. And NNT pathophysiological roles after the discovery of a spontaneous Nnt mutation in C57BL/6J mice. And Nnt silencing reduced the growth of cancer cell lines, suggesting that NNT might be a therapeutic target in some cancers.

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NAD(P) transhydrogenase/NNT Protein, Human, Recombinant (His)

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