Murine polyomavirus (MPyV) (strain Kilham) VP1 Protein (His)

Murine polyomavirus (MPyV) (strain Kilham) VP1 Protein (His) is expressed in Baculovirus insect cells with C-6xHis tag. The predicted molecular weight is 42.5 kDa and the accession number is P24595.

规格	价格/CNY		数量
20 μg	￥ 3,120	20日内发货
100 μg	￥ 8,760	20日内发货
500 μg	￥ 12,500	20日内发货

规格

价格/CNY

货期

数量

20 μg

￥ 3,120

20日内发货

100 μg

￥ 8,760

20日内发货

500 μg

￥ 12,500

20日内发货

产品描述	Murine polyomavirus (MPyV) (strain Kilham) VP1 Protein (His) is expressed in Baculovirus insect cells with C-6xHis tag. The predicted molecular weight is 42.5 kDa and the accession number is P24595.

种属	MPyV
表达系统	Baculovirus
标签	C-terminal 6xHis-tagged
蛋白编号	P24595
氨基酸序列	APTVKKRTSQNQGLSPQKSQNSVVVGGIQVLDVRTGPDSITQIEAFLNPRMGKPVDSDFYGFSDNITVSADYTQDMPRIKELPCYSMAKISLPMLNEDMTCDTILMWEAISCKTEVVGVSSLTNCHSAVKRLYDNEGAGFPVQGLNFHFFSVGGEALDLQWLWKNYRCNYPAGVAALQAAPKAAQVLDPKLKAKLTADGKFPIEAWSPDPAKNENTRYFGTYTGGLQTPPVLQITNTTTTILLNENGVGPLCKGDGLYLASADIVGFRTQQNNKMHLRGLPRYFSIHLRKGCANPYPVSSLLNTFSSEMMPLNSWMLQVEEVRIYDGVERLPGDPDMIRYRIIWPGRLLSLIFPAMRHKHLYFFVMQAFIVL Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
蛋白构建	2-373 aa
蛋白纯度	> 85% as determined by SDS-PAGE.
分子量	42.5 kDa (predicted)

缓冲液	If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
复溶方法	A hardcopy of COA with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
存储	Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
运输方式	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
研究背景	Forms an icosahedral capsid with a T=7 symmetry and a 40 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with VP2 or VP3 proteins. Interacts with terminal alpha(2,3)-linked sialic acids on the cell surface to provide virion attachment to target cell. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. Once attached, the virion is internalized by caveolin-mediated endocytosis and traffics to the endoplasmic reticulum. Inside the endoplasmic reticulum, the protein folding machinery isomerizes VP1 interpentamer disulfide bonds, thereby triggering initial uncoating. Next, the virion uses the endoplasmic reticulum-associated degradation machinery to probably translocate in the cytosol before reaching the nucleus. Nuclear entry of the viral DNA involves the selective exposure and importin recognition of VP2/Vp3 nuclear localization signal. In late phase of infection, neo-synthesized VP1 encapsulates replicated genomic DNA in the nucleus, and participates in rearranging nucleosomes around the viral DNA.

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Murine polyomavirus (MPyV) (strain Kilham) VP1 Protein (His)

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