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MGMT Protein, Human, Recombinant (His)

MGMT Protein, Human, Recombinant (His)

产品编号 TMPJ-00872
别名: MGMT, 6-O-methylguanine-DNAmethyltransferase, O-6-methylguanine-DNA-alkyltransferase, Methylated-DNA--protein-cysteine methyltransferase

MGMT belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. MGMT involved in the cellular defense against the biological effects of O6-methylguanine in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. MGMT catalyzes the chemical reaction: DNA (containing 6-O-methylguanine) and proteinL-cysteine into DNA (without 6-O-methylguanine) and protein S-methyl-L-cysteine.

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MGMT Protein, Human, Recombinant (His)
规格 价格/CNY 货期 数量
10 μg ¥ 740 5日内发货
50 μg ¥ 2,240 5日内发货
500 μg ¥ 12,100 5日内发货
1 mg ¥ 17,400 5日内发货
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产品目录号及名称: MGMT Protein, Human, Recombinant (His) (TMPJ-00872)
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产品性质
产品描述 MGMT belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. MGMT involved in the cellular defense against the biological effects of O6-methylguanine in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. MGMT catalyzes the chemical reaction: DNA (containing 6-O-methylguanine) and proteinL-cysteine into DNA (without 6-O-methylguanine) and protein S-methyl-L-cysteine.
种属 Human
表达系统 E. coli
标签 N-6His
蛋白编号 P16455
别名 MGMT, 6-O-methylguanine-DNAmethyltransferase, O-6-methylguanine-DNA-alkyltransferase, Methylated-DNA--protein-cysteine methyltransferase
氨基酸序列 Met1-Asn207
蛋白构建 Recombinant Human Methylated-DNA-protein-cysteine Methyltransferase is produced by our E.coli expression system and the target gene encoding Met1-Asn207 is expressed with a 6His tag at the N-terminus.
蛋白纯度 Greater than 90% as determined by reducing SDS-PAGE. (QC verified)
分子量 25 KDa, reducing conditions
内毒素 Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
缓冲液 Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl,1mM DTT,1mM EDTA,500mM NaCl,0.1%Trition X-100,pH 8.0.
存储

Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

运输方式

The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.

研究背景 MGMT belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. MGMT involved in the cellular defense against the biological effects of O6-methylguanine in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. MGMT catalyzes the chemical reaction: DNA (containing 6-O-methylguanine) and proteinL-cysteine into DNA (without 6-O-methylguanine) and protein S-methyl-L-cysteine.

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Keywords

MGMT Protein, Human, Recombinant (His) MGMT 6-O-methylguanine-DNAmethyltransferase O-6-methylguanine-DNA-alkyltransferase Methylated-DNA--protein-cysteine methyltransferase recombinant recombinant-proteins proteins protein

 

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