Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA0

Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin/HA0 Protein

产品编号 TMPY-04293 Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin/HA0 Protein

Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin/HA0 Protein is expressed in Baculovirus insect cells. The predicted molecular weight is 61.9 kDa and the accession number is C6KNH7.

规格	价格	库存
100 μg	¥ 6,420	现货
200 μg	¥ 10,950	5日内发货
500 μg	¥ 22,130	5日内发货

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常见问题解答

问产品溶解需要超声吗？如果没有超声仪怎么办？

答超声可以加快粉末的溶解速度，如果您的化合物溶解不了，建议您尝试超声。如果您没有超声仪，建议您选择较低母液浓度，或者较少粉末量，尝试溶解。

问准备进行动物实验，收到大包装粉末后，如何分装溶解？

答根据配制出来的溶液是不是澄清的确定一次性能配多少：如果配制出来是澄清溶液，可以一次性多配制一些，储存于4度冰箱，大概一周配一次，放久了可能会失效；如果配制出来是混悬液，建议每次使用的时候都现配现用。

问收到粉状试剂粘壁严重，如何处理？

答建议您用离心机 3000 rpm下离心一会儿,将粉末收集到管底。

问抑制剂是否可以高温灭菌？

答大部分产品均通过化学方法合成，反应条件一般在 50-80℃ 之间，因此不建议使用高温灭菌法进行灭菌，建议用 0.22 μm 滤膜过滤灭菌。

问收到化合物后，是否需要称量后配制储备液？

答对于小包装，如 1 mg、5 mg、10 mg 等，不建议二次称量。这样会导致化合物损耗，且无法估算。TargetMol 所有产品原装进口，精准称量，您收到后可直接添加相应量溶剂。对于大包装，如果您需要分批次实验，可每次称量后配制储备液，建议您 10 mg 起称，称量的误差会小一些；当然条件允许，也可以先配制成高浓度母液，分装备用。

产品信息

生物活性	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述	Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin/HA0 Protein is expressed in Baculovirus insect cells. The predicted molecular weight is 61.9 kDa and the accession number is C6KNH7.
种属	H3N2
表达系统	Baculovirus Insect Cells
标签	Tag Free
蛋白编号	C6KNH7
别名	Harvey rat sarcoma viral oncogene homolog
蛋白构建	A DNA sequence encoding full length of the Influenza A virus (A/Perth/16/2009(H3N2)) hemagglutinin (ACS71642.1) (Met1-Ile566), termed as HA, was expressed. Predicted N terminal: Gln 17
蛋白纯度	> 85 % as determined by SDS-PAGE.
分子量	61.9 kDa (predicted)
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 150 mM NaCl, pH 7.5, 5% glycerol. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.

Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin/HA0 Protein

Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin/HA0 Protein

产品信息

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