IL-18 Protein, Mouse, Recombinant (His)

Interleukin-18 (IL-18）is a protein which belongs to the IL-1 family. It is expressed as a 24 kDa precursor by endothelial and epithelial cells, keratinocytes, gamma δ T cells, and phagocytes. Mature mouse IL-18 shares 63% and 91% amino acid sequence identity with mouse and rat IL-18, respectively. IL-18 binds to the widely expressed IL-18 R alpha which recruits IL-18 R beta to form the signaling receptor complex. Its bioactivity is negatively regulated by interactions with IL-18 binding proteins and virally encoded IL-18BP homologs. It augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. In the presence of IL-12 or IL-15, IL-18 enhances anti-viral Th1 immune responses by inducing IFN-gamma production and the cytolytic activity of CD8+ T cells and NK cells. In the absence of IL-12 or IL-15, however, IL-18 promotes production of the Th2 cytokines IL-4 and IL-13 by CD4+ T cells and basophils.

规格	价格/CNY		数量
10 μg	￥ 820	5日内发货
50 μg	￥ 2,480	5日内发货
500 μg	￥ 12,100	5日内发货
1 mg	￥ 17,400	5日内发货

规格

价格/CNY

货期

数量

10 μg

￥ 820

5日内发货

50 μg

￥ 2,480

5日内发货

500 μg

￥ 12,100

5日内发货

1 mg

￥ 17,400

5日内发货

产品描述

种属	Mouse
表达系统	E. coli
标签	N-6His
蛋白编号	P70380
别名	Interferon γ-inducing factor, Interleukin-1 gamma, Igif, IL-1 gamma, Interleukin-1 γ, IL-1 γ, Interferon gamma-inducing factor, Il18, 白介素, 白细胞介素, IFN-γ-inducing factor, IFN-gamma-inducing factor, Interleukin-18
氨基酸序列	Asn36-Ser192
蛋白构建	Recombinant Mouse Interleukin-18 is produced by our E.coli expression system and the target gene encoding Asn36-Ser192 is expressed with a 6His tag at the N-terminus.
蛋白纯度	Greater than 95% as determined by reducing SDS-PAGE. Greater than 95% as determined by SEC-HPLC.
分子量	20 KDa, reducing conditions

内毒素	Less than 1 EU/µg as determined by LAL test.
缓冲液	Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 10% Trehalose, 50mM NaCl, 0.1mM EDTA, 0.05% Tween 80, pH 8.0.
复溶方法	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
存储	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
运输方式	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
研究背景	Interleukin-18 (IL-18）is a protein which belongs to the IL-1 family. It is expressed as a 24 kDa precursor by endothelial and epithelial cells, keratinocytes, gamma δ T cells, and phagocytes. Mature mouse IL-18 shares 63% and 91% amino acid sequence identity with mouse and rat IL-18, respectively. IL-18 binds to the widely expressed IL-18 R alpha which recruits IL-18 R beta to form the signaling receptor complex. Its bioactivity is negatively regulated by interactions with IL-18 binding proteins and virally encoded IL-18BP homologs. It augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. In the presence of IL-12 or IL-15, IL-18 enhances anti-viral Th1 immune responses by inducing IFN-gamma production and the cytolytic activity of CD8+ T cells and NK cells. In the absence of IL-12 or IL-15, however, IL-18 promotes production of the Th2 cytokines IL-4 and IL-13 by CD4+ T cells and basophils.

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IL-18 Protein, Mouse, Recombinant (His)

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