IL-18 Protein, Human, Recombinant

Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1-like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cell -, IL-4- and STAT6-dependent fashion. IL-18 and T cell receptor-mediated stimulation could induce naive CD4+ T cells to develop into IL-4-producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in the regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-α and IL-1β, suggesting that monokine expression can feedback to promote Th1 cell development in the synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

规格	价格/CNY		数量
10 μg	￥ 990	现货
20 μg	￥ 1,570	5日内发货
50 μg	￥ 2,650	5日内发货
100 μg	￥ 3,930	5日内发货
200 μg	￥ 6,910	5日内发货

规格

价格/CNY

货期

数量

10 μg

￥ 990

现货

20 μg

￥ 1,570

5日内发货

50 μg

￥ 2,650

5日内发货

100 μg

￥ 3,930

5日内发货

200 μg

￥ 6,910

5日内发货

生物活性

1.Measured by its ability to induce IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells in the presence of TNF-alpha. The ED50 for this effect is typically 5-30 ng/ml. 2.Loaded Recombinant Human IL-18R beta / IL-18RAP Protein, Fc Tag on ProA Biosensor, can bind Recombinant Human IL-18Rα Protein, His Tag (Premixed with Human IL-18, Tag Free) with an affinity constant of 7.7nM as determined in BLI assay (Routinely tested).

TargetMol Bioactive

产品描述

种属	Human
表达系统	E. coli
标签	Tag Free
蛋白编号	Q14116-1
别名	IGIF, IL-18, IL1F4, IL-1g, Interleukin 18
蛋白构建	A DNA sequence encoding the human IL18 (NP_001553.1) (Tyr37-Asp193) was expressed.
蛋白纯度	> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
分子量	Approxiamtely 18.2 kDa

内毒素	< 10 EU per mg of the protein as determined by the LAL method.
缓冲液	Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
复溶方法	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
存储	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
运输方式	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
研究背景	Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1-like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cell -, IL-4- and STAT6-dependent fashion. IL-18 and T cell receptor-mediated stimulation could induce naive CD4+ T cells to develop into IL-4-producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in the regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-α and IL-1β, suggesting that monokine expression can feedback to promote Th1 cell development in the synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy