IL-10R alpha/IL-10RA Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 27 kDa and the accession number is Q13651.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 3,100 | 5日内发货 | ||
500 μg | ¥ 12,400 | 5日内发货 | ||
1 mg | ¥ 20,700 | 5日内发货 |
生物活性 | 1. Immobilized Human IL-10, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IL-10 R alpha, His Tag with the EC50 of 21.8ng/ml determined by ELISA (QC Test). 2. Human IL-10 R alpha, His Tag immobilized on CM5 Chip can bind Human IL-10, His Tag with an affinity constant of 0.13 nM as determined in SPR assay. |
产品描述 | IL-10R alpha/IL-10RA Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 27 kDa and the accession number is Q13651. |
种属 | Human |
表达系统 | HEK293 |
标签 | C-His-Avi |
蛋白编号 | Q13651 |
别名 | CDw210a, IL-10 R alpha, IBD28, IL-10 R α, IL10RA, IL-10RA, IL-10R subunit 1, IL10R, HIL-10R, IL-10R1 |
蛋白构建 | Recombinant Human IL-10 R alpha Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains His22-Asn235.[Accession |Q13651] |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | The protein has a predicted MW of 27 kDa. Due to glycosylation, the protein migrates to 40-48 kDa based on Tris-Bis PAGE result. |
内毒素 | Less than 1EU per μg by the LAL method. |
缓冲液 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
复溶方法 | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
存储 |
-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
运输方式 |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
研究背景 | The interleukin-10 receptor alpha (IL10RA) gene codes for the alpha chain of the IL-10 receptor which binds the cytokine IL-10. IL-10 is an anti-inflammatory cytokine with immunoregulatory function during the pathogenesis of many inflammatory disorders in livestock, including Johne's disease (JD). JD is a chronic enteritis in cattle caused by Mycobacterium avium subsp. paratuberculosis (MAP) and is responsible for significant economic losses to the dairy industry. Several candidate genes including IL10RA have been found to be associated with JD. |
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IL-10R alpha/IL-10RA Protein, Human, Recombinant (His & Avi) IBD 28 CDw210a IL-10 R alpha IBD28 IL-10 R α IL10RA IL-10RA IL-10R subunit 1 IL10R HIL-10R IL-10R1 IBD-28 recombinant recombinant-proteins proteins protein