IL-10 Protein, Human, Recombinant (His & Avi)

Interleukin 10 (IL10) is a key anti-inflammatory cytokine that can inhibit proinflammatory responses of both innate and adaptive immune cells. An association between IL10 and intestinal mucosal homeostasis became clear with the discovery that IL10 and IL10 receptor (IL10R)-deficient mice develop spontaneous intestinal inflammation. Similarly, patients with deleterious mutations in IL10, IL10RA, or IL10RB present with severe enterocolitis within the first months of life. IL-10 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with N-His-Avi tag. The predicted molecular weight is 21.3 kDa and the accession number is P22301.

规格	价格/CNY		数量
100 μg	￥ 4,440	5日内发货
500 μg	￥ 17,800	5日内发货
1 mg	￥ 29,600	5日内发货

规格

价格/CNY

货期

数量

100 μg

￥ 4,440

5日内发货

500 μg

￥ 17,800

5日内发货

1 mg

￥ 29,600

5日内发货

生物活性

1. Immobilized Human IL-10, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-10R alpha, His Tag with the EC50 of 28.7ng/ml determined by ELISA. 2. Human IL-10R alpha, His Tag immobilized on CM5 Chip can bind Human IL-10, His Tag with an affinity constant of 0.13 nM as determined in SPR assay.

产品描述

种属	Human
表达系统	HEK293
标签	N-His-Avi
蛋白编号	P22301
别名	MGC126450, 白细胞介素, GVHDS, B-TCGF, IL10A, 白介素, TGIF, CSIF, IL-10, Interleukin-10, MGC126451
蛋白构建	Recombinant Human IL-10 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus. It contains Ser19-Asn178.[Accession \|P22301]
蛋白纯度	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	The protein has a predicted MW of 21.3 kDa. Due to glycosylation, the protein migrates to 23-25 kDa based on Tris-Bis PAGE result.

内毒素	Less than 1EU per μg by the LAL method.
缓冲液	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶方法	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
存储	-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
运输方式	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
研究背景	Interleukin 10 (IL10) is a key anti-inflammatory cytokine that can inhibit proinflammatory responses of both innate and adaptive immune cells. An association between IL10 and intestinal mucosal homeostasis became clear with the discovery that IL10 and IL10 receptor (IL10R)-deficient mice develop spontaneous intestinal inflammation. Similarly, patients with deleterious mutations in IL10, IL10RA, or IL10RB present with severe enterocolitis within the first months of life.