IL-36 alpha/IL-1F6 Protein, Human, Recombinant (aa 6-158)

Interleukin-1 family member 6 (IL-1F6), also known as interleukin 36, alpha (IL36A), is a pro-inflammatory cytokine that plays an important role in innate and adaptive immunity. IL-1F6 activates MAPK and NF-kB pathways and is produced by many different cells. This cytokine is a family member of interleukin-1 (IL-1) and plays an important role in the pathophysiology of several diseases. It has been reported that IL-1F6 and IL-1F8, in addition to IL-1F9, activate the pathway leading to NF-kappaB in an IL-1Rrp2-dependent manner in Jurkat cells as well as in multiple other human and mouse cell lines. Activation of the pathway leading to NF-kappaB by IL-1F6 and IL-1F8 follows a similar time course to activation by IL-1beta, suggesting that signaling by the novel family members occurs through a direct mechanism. In a mammary epithelial cell line, NCI/ADR-RES, which naturally expresses IL-1Rrp2, all three cytokines signal without further receptor transfection. IL-1Rrp2 antibodies block activation of the pathway leading to NF-kappaB by IL-1F6, IL-1F8, and IL-1F9 in both Jurkat and NCI/ADR-RES cells. Thus IL-1F6, IL-1F8, and IL-1F9 signal through IL-1Rrp2 and IL-1RAcP.

规格	价格/CNY		数量
20 μg	￥ 1,950	现货
100 μg	￥ 6,740	5日内发货
200 μg	￥ 11,490	5日内发货
500 μg	￥ 23,220	5日内发货

规格

价格/CNY

货期

数量

20 μg

￥ 1,950

现货

100 μg

￥ 6,740

5日内发货

200 μg

￥ 11,490

5日内发货

500 μg

￥ 23,220

5日内发货

生物活性

Measured by its ability to induce IL8 secretion in A431 human epithelial carcinoma cells. The ED50 for this effect is typically 5-20 ng/mL.

TargetMol Bioactive

产品描述

种属	Human
表达系统	E. coli
标签	Tag Free
蛋白编号	Q9UHA7
别名	interleukin 36, alpha, 白细胞介素, FIL1(ε), IL1(ε), 白介素, IL1E, IL-1F6, FIL1E, IL1F6, IL1(EPSILON), FIL1, FIL1(EPSILON), IL36 α/IL-1F6, IL36A, FIL, 1E, interleukin 36, α
蛋白构建	A DNA sequence encoding the human IL1F6 (Q9UHA7) (Ala6-Phe158) was expressed and purified, with two additional amino acids (Gly & Pro) at the N-terminus.
蛋白纯度	> 95 % as determined by SDS-PAGE
分子量	17 .2 kDa (predicted)

内毒素	Please contact us for more information.
缓冲液	Lyophilized from sterile PBS pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
复溶方法	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
存储	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
运输方式	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
研究背景	Interleukin-1 family member 6 (IL-1F6), also known as interleukin 36, alpha (IL36A), is a pro-inflammatory cytokine that plays an important role in innate and adaptive immunity. IL-1F6 activates MAPK and NF-kB pathways and is produced by many different cells. This cytokine is a family member of interleukin-1 (IL-1) and plays an important role in the pathophysiology of several diseases. It has been reported that IL-1F6 and IL-1F8, in addition to IL-1F9, activate the pathway leading to NF-kappaB in an IL-1Rrp2-dependent manner in Jurkat cells as well as in multiple other human and mouse cell lines. Activation of the pathway leading to NF-kappaB by IL-1F6 and IL-1F8 follows a similar time course to activation by IL-1beta, suggesting that signaling by the novel family members occurs through a direct mechanism. In a mammary epithelial cell line, NCI/ADR-RES, which naturally expresses IL-1Rrp2, all three cytokines signal without further receptor transfection. IL-1Rrp2 antibodies block activation of the pathway leading to NF-kappaB by IL-1F6, IL-1F8, and IL-1F9 in both Jurkat and NCI/ADR-RES cells. Thus IL-1F6, IL-1F8, and IL-1F9 signal through IL-1Rrp2 and IL-1RAcP.