IgE Protein, Human, Recombinant (His & Avi)

Immunoglobulin E (IgE) is well known for its role in allergic disease, the manifestations of which are mediated through its two Fc receptors, FcεRI and CD23 (FcεRII). IgE and its interactions with these receptors are therefore potential targets for therapeutic intervention, and exciting progress has been made in this direction. Furthermore, recent structural studies of IgE-Fc, the two receptors, and of their complexes, have revealed a remarkable degree of plasticity at the IgE-CD23 interface and an even more remarkable degree of dynamic flexibility within the IgE molecule.

规格	价格/CNY		数量
100 μg	￥ 3,700	现货
500 μg	￥ 14,800	5日内发货
1 mg	￥ 24,700	5日内发货

规格

价格/CNY

货期

数量

100 μg

￥ 3,700

现货

500 μg

￥ 14,800

5日内发货

1 mg

￥ 24,700

5日内发货

生物活性

Immobilized Human IgE, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-IgE Antibody, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA.

产品描述

种属	Human
表达系统	HEK293
标签	C-His-Avi
蛋白编号	P01854
别名	Ig ε chain C region, IgE, Ig epsilon chain C region
蛋白构建	Recombinant Human IgE Protein is expressed from HEK293 with His and Avi tag at the C-Terminus. It contains Cys209-Lys428.[Accession \|P01854]
蛋白纯度	> 95% as determined by Tris-Bis PAGE
分子量	The protein has a predicted MW of 27.8 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result.

内毒素	Less than 1EU per μg by the LAL method.
缓冲液	Lyophilized from 0.22μm filtered solution in 20mM PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶方法	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
存储	-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
运输方式	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
研究背景	Immunoglobulin E (IgE) is well known for its role in allergic disease, the manifestations of which are mediated through its two Fc receptors, FcεRI and CD23 (FcεRII). IgE and its interactions with these receptors are therefore potential targets for therapeutic intervention, and exciting progress has been made in this direction. Furthermore, recent structural studies of IgE-Fc, the two receptors, and of their complexes, have revealed a remarkable degree of plasticity at the IgE-CD23 interface and an even more remarkable degree of dynamic flexibility within the IgE molecule.