At least 23 different variants of IFN-α are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-α subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-α subtypes differ in their sequences by only one or two positions. Naturally occurring variants also include proteins that are truncated by 10 amino acids at the carboxyl-terminal end.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
10 μg | ¥ 380 | 现货 | ||
20 μg | ¥ 590 | 5日内发货 | ||
50 μg | ¥ 1,090 | 5日内发货 | ||
100 μg | ¥ 1,470 | 5日内发货 |
生物活性 |
1. Loaded Human IFNAR2-Fc on Protein A Biosensor, can bind Human IFN alpha2b with an affinity constant of 2.98 nM as determined in BLI assay. (Regularly tested)
2. Measured in antiviral assay using A549 human lung cancer cells infected with vesicular stomatitisvirus (VSV) The ED50 for this effect is 5 ng/mL. (Regularly tested)
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产品描述 | At least 23 different variants of IFN-α are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-α subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-α subtypes differ in their sequences by only one or two positions. Naturally occurring variants also include proteins that are truncated by 10 amino acids at the carboxyl-terminal end. |
种属 | Human |
表达系统 | E. coli |
标签 | None |
蛋白编号 | P01563 |
别名 | 干扰素, IFNA2, Interferon α-2, Interferon α-A, Interferon Alpha-2, IFN-α-2, LeIF A, Interferon Alpha-A, IFN-Alpha-2, IFN α2b |
氨基酸序列 | Cys24-Glu188 |
蛋白构建 | Recombinant Human Interferon Alpha-2b is produced by our E.coli expression system and the target gene encoding Cys24-Glu188 is expressed. |
蛋白纯度 |
Greater than 95% as determined by reducing SDS-PAGE. Greater than 95% as determined by SEC-HPLC.
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分子量 | 17 KDa, reducing conditions |
内毒素 | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
缓冲液 | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
复溶方法 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
存储 |
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
运输方式 |
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. |
研究背景 | At least 23 different variants of IFN-α are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-α subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-α subtypes differ in their sequences by only one or two positions. Naturally occurring variants also include proteins that are truncated by 10 amino acids at the carboxyl-terminal end. |
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IFN-alpha 2b/IFNA2 Protein, Human, Recombinant 干扰素 Interferon a-2 IFNA2 Interferon α-2 Interferon α-A Interferon Alpha-2 IFNA 2 IFN-α-2 IFN-a-2 LeIF A Interferon Alpha-A IFNA-2 IFN-Alpha-2 IFN α2b Interferon a-A recombinant recombinant-proteins proteins protein