HER3/ERBB3 Protein, Human, Recombinant (aa 730-1065, His & GST)

ErbB3, also known as Her3(human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

规格	价格/CNY		货期	数量
50 μg	￥ 4,460	5日内发货

规格

价格/CNY

货期

数量

50 μg

￥ 4,460

5日内发货

生物活性

1. No Kinase Activity 2. Using the Octet RED System, the affinity constant (Kd) of human ErBB3-GST bound to human NRG1 (aa 2-224) was 20 nM.

产品描述

种属	Human
表达系统	Baculovirus-Insect Cells
标签	His,GST
蛋白编号	P21860-1
别名	c-erbB3, ErbB-3, p45-sErbB3, LCCS2, p85-sErbB3, HER3, EEBB3, MDA-BF-1, p180-ErbB3, c-erbB-3, erbB3-S, erb-b2 receptor tyrosine kinase 3
蛋白构建	A DNA sequence encoding the cytoplasmic domain (Pro 730-Ser 1065) of human ErbB3 (NP_001973.2) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
蛋白纯度	> 75 % as determined by SDS-PAGE
分子量	Approxiamtely 65 kDa

内毒素	< 1.0 EU per μg of the protein as determined by the LAL method
缓冲液	Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 7.5, 10% gly, 1mM GSHPlease contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
复溶方法	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
存储	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
运输方式	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
研究背景	ErbB3, also known as Her3(human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy