ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors.Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 5,180 | 5日内发货 | ||
500 μg | ¥ 20,700 | 5日内发货 | ||
1 mg | ¥ 34,600 | 5日内发货 |
生物活性 | Immobilized Anti-HER2 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Her2 Domain 4, His Tag with the EC50 of 0.82μg/ml determined by ELISA. |
产品描述 | ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors.Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. |
种属 | Human |
表达系统 | HEK293 |
标签 | C-His-Avi |
蛋白编号 | P04626-1 |
别名 | MLN19, HERV7Q, HERVW, MLN 19, TKR1, herstatin, ENVW, EGFR2, ERBB2, ERVWE1, NGL, HER2, ENV, HER-2, CD340, NEU |
蛋白构建 | Recombinant Biotinylated Human Her2/ErbB2 Domain 4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Pro489-Cys630.[Accession |P04626-1] |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | The protein has a predicted MW of 18.5 kDa. Due to glycosylation, the protein migrates to 28-40 kDa based on Tris-Bis PAGE result. |
内毒素 | Less than 1EU per μg by the LAL method. |
缓冲液 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
复溶方法 | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
存储 |
-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
运输方式 |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
研究背景 | ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors.Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. |
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HER2/ERBB2 Domain 4 Protein, Human, Recombinant (His & Avi), Biotinylated TKR-1 MLN19 HERV7Q HERVW ERVWE-1 MLN-19 MLN 19 EGFR 2 TKR1 ERVWE 1 herstatin ENVW EGFR2 ERBB2 ERVWE1 CD-340 EGFR-2 NGL HER2 TKR 1 ENV CD 340 HER-2 HER 2 CD340 ERBB-2 ERBB 2 NEU recombinant recombinant-proteins proteins protein