HER2/ERBB2 Domain 4 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 18.5 kDa and the accession number is P04626-1.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 2,960 | 5日内发货 | ||
500 μg | ¥ 11,800 | 5日内发货 | ||
1 mg | ¥ 19,700 | 5日内发货 |
生物活性 | Immobilized Human Her2 Domain 4, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-Her2 Antibody, hFc Tag with the EC50 of 13.1ng/ml determined by ELISA. |
产品描述 | HER2/ERBB2 Domain 4 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 18.5 kDa and the accession number is P04626-1. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-His-Avi |
蛋白编号 | P04626-1 |
别名 | HERV7Q, HER-2, HER2, NGL, EGFR2, ENVW, NEU, MLN 19, HERVW, CD340, ErbB2, ERVWE1, TKR1, ENV, MLN19, herstatin |
蛋白构建 | Pro489-Cys630 |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | 18.5 kDa (predicted). Due to glycosylation, the protein migrates to 28-40 kDa based on Tris-Bis PAGE result. |
内毒素 | < 1 EU/μg by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors.Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. |
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HER2/ERBB2 Domain 4 Protein, Human, Recombinant (His & Avi) ERVWE-1 HERV7Q ERVWE 1 HER-2 TKR-1 CD-340 TKR 1 HER2 NGL EGFR2 ENVW EGFR-2 NEU MLN 19 HERVW CD340 CD 340 ErbB2 ERVWE1 HER 2 TKR1 MLN-19 ENV MLN19 herstatin EGFR 2 recombinant recombinant-proteins proteins protein