Orphan G-protein-coupled receptor family C group 5 member D (GPRC5D) has seven transmembrane segments forming the core of the receptor, as well as an intracellular C-tail and an extracellular N-terminal domain. GPRC5D is highly and selectively expressed on the surface of CD138+ multiple myeloma (MM) cells, whereas in normal tissues, a much lower expression is limited to plasma cells and certain hard keratinizing tissues. This makes GPRC5D a promising marker for monitoring tumor load and a target antigen for MM-specific T cell-engaging therapies such as bispecific antibodies and CAR-T cells.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
20 μg | ¥ 7,680 | 5日内发货 | ||
100 μg | ¥ 26,900 | 5日内发货 |
生物活性 | 1. Immobilized Human GPRC5D Protein at 5 μg/mL (100 μL/well) on Nickel coated plate can bind Anti-Human GPRC5D Antibody, Human IgG1, the EC50 is 4.0 ng/ml(QC tested). 2.Loaded Anti-GPRC5D Antibody on Protein A Biosensor, can bind GPRC5D Protein with an affinity constant of 58.1 nM as determined in BLI assay (Routinely tested). |
产品描述 | Orphan G-protein-coupled receptor family C group 5 member D (GPRC5D) has seven transmembrane segments forming the core of the receptor, as well as an intracellular C-tail and an extracellular N-terminal domain. GPRC5D is highly and selectively expressed on the surface of CD138+ multiple myeloma (MM) cells, whereas in normal tissues, a much lower expression is limited to plasma cells and certain hard keratinizing tissues. This makes GPRC5D a promising marker for monitoring tumor load and a target antigen for MM-specific T cell-engaging therapies such as bispecific antibodies and CAR-T cells. |
种属 | Human |
表达系统 | HEK293 |
标签 | His,FLAG |
蛋白编号 | Q9NZD1 |
别名 | GPRC5D |
蛋白构建 | A DNA sequence encoding the human GPRC5D (Q9NZD1) (Met1-Ser268) was expressed, with a Flag tag at the N-terminus and a polyhistidine tag at the C-terminus. |
蛋白纯度 | ≥ 80 % as determined by SDS-PAGE. |
分子量 | 32.83 kDa (predicted) |
内毒素 | < 1.0 EU per μg protein as determined by the LAL method. |
缓冲液 | Supplied as solution in 10mM HEPES, 50mM NaCl, DDM, pH7.5 with glycerol as protectant. DDM is cytotoxicity and not suitable for cell based experiments. Never remove DDM as it is necessary to the activity of the protein. Do not store the protein without glycerol. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA. |
存储 |
Samples are stable for up to three months from date of receipt at -70℃. Store it under sterile conditions at -70℃ or lower. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
运输方式 |
It is supplied and shipped as Solution with dry ice. |
研究背景 | Orphan G-protein-coupled receptor family C group 5 member D (GPRC5D) has seven transmembrane segments forming the core of the receptor, as well as an intracellular C-tail and an extracellular N-terminal domain. GPRC5D is highly and selectively expressed on the surface of CD138+ multiple myeloma (MM) cells, whereas in normal tissues, a much lower expression is limited to plasma cells and certain hard keratinizing tissues. This makes GPRC5D a promising marker for monitoring tumor load and a target antigen for MM-specific T cell-engaging therapies such as bispecific antibodies and CAR-T cells. |
bottom
您可能有的问题的答案可以在重组蛋白操作手册中找到
GPRC5D Protein, Human, Recombinant (His & FLAG), Detergent GPRC5D recombinant recombinant-proteins proteins protein